Professor Rainer Cramer

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+44 (0) 118 378 4550
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Professor
- Alexander-von-Humboldt Fellow
- Professor of Mass Spectrometry and Bioanalytical Sciences
- Head of Biomedical, Molecular and Analytical Chemistry.
Office
123Building location
ChemistryAreas of interest
Professor Cramer is an interdisciplinary researcher, who started his academic career as an experimental physicist and later moved on to cancer research and other research fields in the life sciences. He has a strong background in proteomics, biological mass spectrometry and analytical chemistry.In general, his research interests are focused on the application of his physical science expertise to the analytical challenges posed by modern biosciences. He also has a strong interest in the fundamentals of mass spectrometry, particularly MALDI MS.
Read more about Professor Cramer's research interests
Read about Professor Cramer's professional activities
Teaching
Learn about Professor Cramer's teaching.Research centres and groups
Professor Cramer is part of the Biomedical, Molecular and Analytical Chemistry research group and Professor Cramer's research group.
Learn more about our research groupsWebsites/blogs
Visit Professor Cramer's research group's Twitter and LinkedIn pages, and their project website.Publications
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Krenkel, H.
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Brown, J.
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Morris, M.
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Cramer, R.
ORCID: 0000-0002-8037-2511 (2023) The use of salts, buffers and surfactants in LAP-MALDI MS. International Journal of Mass Spectrometry ISSN: 1387-3806 | doi: https://dx.doi.org/10.1016/j.ijms.2023.117134
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Lellman, S.
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Reynolds, C.
ORCID: 0000-0002-4152-1190 , Jones, A. , Taylor, N. , Cramer, R.
ORCID: 0000-0002-8037-2511 (2023) LAP-MALDI MS profiling and identification of potential biomarkers for the detection of bovine tuberculosis. Journal of Agricultural and Food Chemistry , 71 (37). pp. 13899-13905. ISSN: 1520-5118 | doi: https://dx.doi.org/10.1021/acs.jafc.3c01879
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Challen, B.
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Morris, M.
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Cramer, R.
ORCID: 0000-0002-8037-2511 (2023) Ultra-high-throughput and low-volume analysis of intact proteins with LAP-MALDI MS. Journal of the American Society for Mass Spectrometry , 34 (6). pp. 991-994. ISSN: 1879-1123 | doi: https://dx.doi.org/10.1021/jasms.3c00068
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Piras, C.
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Cramer, R.
ORCID: 0000-0002-8037-2511 (2023) Liquid Atmospheric Pressure (LAP) - MALDI MS(/MS) biomolecular profiling for large-scale detection of animal disease and food adulteration and bacterial identification. In: Shah, H. , Gharbia, S. , Shah, A. , Tranfield, E. , Thompson, C. , (eds.) Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry: Industrial and Environmental Applications. Wiley ISBN: 9781119814054
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Challen, B.
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Cramer, R.
ORCID: 0000-0002-8037-2511 (2022) Advances in ionisation techniques for mass spectrometry‐based omics research. Proteomics , 22 (15-16). pp 0. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.202100394
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Krenkel, H.
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Brown, J.
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Richardson, K.
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Hoyes, E.
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Morris, M.
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Cramer, R.
ORCID: 0000-0002-8037-2511 (2022) Ultrahigh-throughput sample analysis using liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry. Analytical Chemistry , 94 (10). pp. 4141-4145. ISSN: 0003-2700 | doi: https://dx.doi.org/10.1021/acs.analchem.1c05614
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Piras, C.
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Hale, O.
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Reynolds, C.
ORCID: 0000-0002-4152-1190 , Jones, A. , Taylor, N. , Morris, M. , Cramer, R.
ORCID: 0000-0002-8037-2511 (2022) LAP-MALDI MS coupled with machine learning: an ambient mass spectrometry approach for high-throughput diagnostics. Chemical Science , 13 (6). pp. 1746-1758. ISSN: 1478-6524 | doi: https://dx.doi.org/10.1039/D1SC05171G
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Piras, C.
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Hale, O.
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Reynolds, C.
ORCID: 0000-0002-4152-1190 , Jones, A. , Taylor, N. , Morris, M. , Cramer, R.
ORCID: 0000-0002-8037-2511 (2021) Speciation and milk adulteration analysis by rapid ambient liquid MALDI mass spectrometry profiling using machine learning. Scientific Reports , 11 (1). ISSN: 2045-2322 | doi: https://dx.doi.org/10.1038/s41598-021-82846-5
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Piras, C.
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Greco, V.
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Gugliandolo, E.
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Soggiu, A.
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Tilocca, B.
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Bonizzi, L.
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Zecconi, A.
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Cramer, R.
ORCID: 0000-0002-8037-2511 , Britti, D. , Urbani, A. , Roncada, P. (2020) Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (AMR). Animal Feed Science and Technology , 10 (12). ISSN: 0377-8401 | doi: https://dx.doi.org/10.3390/ani10122378
- Piras, C. , Ceniti, C. , Hartmane, E. , Costanzo, N. , Morittu, V. , Roncada, P. , Britti, D. , Cramer, R. (2020) Rapid liquid AP-MALDI MS profiling of lipids and proteins from goat and sheep milk for speciation and colostrum analysis. Proteomes , 8 (3). ISSN: 2227-7382 | doi: https://dx.doi.org/10.3390/proteomes8030020
- Cramer, R. (2020) High-speed analysis of large sample sets – how can this key aspect of the omics be achieved?. Molecular and Cellular Proteomics , 19 (11). pp. 1760-1766. ISSN: 1535-9484 | doi: https://dx.doi.org/10.1074/mcp.P120.001997
- Castelletto, V. , Seitsonen, J. , Ruokolainen, J. , Piras, C. , Cramer, R. , Edwards-Gayle, C. , Hamley, I. (2020) Peptide nanotubes self-assembled from leucine-rich alpha helical surfactant-like peptides. Chemical Communications , 56 (80). pp. 11977-11980. ISSN: 1359-7345 | doi: https://dx.doi.org/10.1039/D0CC04299D
- Scollo, E. , Neville, D. , Oruna-Concha, M. , Trotin, M. , Umaharan, P. , Sukha, D. , Kalloo, R. , Cramer, R. (2020) Proteomic and peptidomic UHPLC-ESI MS/MS analysis of cocoa beans fermented using the Styrofoam-box method. Food Chemistry , 316 ISSN: 0308-8146 | doi: https://dx.doi.org/10.1016/j.foodchem.2020.126350
- Krenkel, H. , Hartmane, E. , Piras, C. , Brown, J. , Morris, M. , Cramer, R. (2020) Advancing liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry toward ultra-high-throughput analysis. Analytical Chemistry , 92 (4). pp. 2931-2936. ISSN: 0003-2700 | doi: https://dx.doi.org/10.1021/acs.analchem.9b05202
- Scollo, E. , Neville, D. , Oruna-Concha, M. , Trotin, M. , Cramer, R. (2020) UHPLC-MS/MS analysis of cocoa bean proteomes from four different genotypes. Food Chemistry , 303 ISSN: 0308-8146 | doi: https://dx.doi.org/10.1016/j.foodchem.2019.125244
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Hale, O.
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Ryumin, P.
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Brown, J.
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Morris, M.
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Cramer, R.
ORCID: 0000-0002-8037-2511 (2020) Production and analysis of multiply charged negative ions by liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry. Rapid Communications in Mass Spectrometry , 35 (S1). ISSN: 1097-0231 | doi: https://dx.doi.org/10.1002/rcm.8246
- Lellman, S. and Cramer, R. (2019) Bacterial identification by lipid profiling using liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry. Clinical Chemistry and Laboratory Medicine , 58 (6). pp. 930-938. ISSN: 1437-4331 | doi: https://dx.doi.org/10.1515/cclm-2019-0908
- Lambertucci, S. , Orman, K. , Das Gupta, S. , Fisher, J. , Gazal, S. , Williamson, R. , Cramer, R. , Bindschedler, L. (2019) Analysis of barley leaf epidermis and extrahaustorial proteomes during powdery mildew infection reveals that the PR5 thaumatin-like protein TLP5 is required for susceptibility towards Blumeria graminis f. sp. hordei. Frontiers in Plant Science , 10 pp 0. ISSN: 1664-462X | doi: https://dx.doi.org/10.3389/fpls.2019.01138
- Hale, O. and Cramer, R. (2019) Atmospheric pressure ultraviolet laser desorption and ionization from liquid samples for native mass spectrometry. Analytical Chemistry , 91 (22). pp. 14192-14197. ISSN: 0003-2700 | doi: https://dx.doi.org/10.1021/acs.analchem.9b03875
- Hale, O. , Morris, M. , Jones, B. , Reynolds, C. , Cramer, R. (2019) Liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry adds enhanced functionalities to MALDI MS profiling for disease diagnostics. ACS Omega , 4 (7). pp. 12759-12765. ISSN: 2470-1343 | doi: https://dx.doi.org/10.1021/acsomega.9b01476
- Hutchinson, J. , Hamley, I. , Edwards-Gayle, C. , Castelletto, V. , Piras, C. , Cramer, R. , Kowalczyk, R. , Seitsonen, J. , Ruokolainen, J. , Rambo, R. (2019) Melanin production by tyrosinase activity on a tyrosine-rich peptide fragment and pH-dependent self-assembly of its lipidated analogue. Organic & Biomolecular Chemistry , 17 pp. 4543-4553. ISSN: 1477-0520 | doi: https://dx.doi.org/10.1039/C9OB00550A
- Ryumin, P. and Cramer, R. (2018) The composition of liquid atmospheric pressure matrix-assisted laser desorption/ionization matrices and its effect on ionization in mass spectrometry. Analytica Chimica Acta , 1013 pp. 43-53. ISSN: 1873-4324 | doi: https://dx.doi.org/10.1016/j.aca.2018.01.070
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Scollo, E.
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Neville, D.
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Oruna-Concha, M.
ORCID: 0000-0001-7916-1592 , Trotin, M. , Cramer, R.
ORCID: 0000-0002-8037-2511 (2018) Characterization of the proteome of Theobroma cacao beans by nano-UHPLC-ESI MS/MS. Proteomics , 18 (3-4). ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.201700339
- Hale, O. and Cramer, R. (2018) Collision-induced dissociation of doubly-charged barium-cationized lipids generated from liquid samples by atmospheric pressure matrix-assisted laser desorption/ionization provides structurally diagnostic product ions. Analytical and Bioanalytical Chemistry , 410 (5). pp. 1435-1444. ISSN: 1618-2650 | doi: https://dx.doi.org/10.1007/s00216-017-0788-6
- Brown, J. , Morris, M. , Cramer, R. (2017) Liquid AP-MALDI: ion signal intensity and persistence at laser repetition rates between 1 Hz and 5 kHz.
- Hale, O. , Morris, M. , Cramer, R. (2017) AP-MALDI-Q-IMS-TOF MS as a liquid sample mass profiling platform for food authenticity tests.
- Torma, A. , Groves, K. , Biesenbruch, S. , Mussell, C. , Reid, A. , Ellison, S. , Cramer, R. , Quaglia, M. (2017) A candidate liquid chromatography mass spectrometry reference method for the quantification of the cardiac marker 1-32 B-type natriuretic peptide. Clinical Chemistry and Laboratory Medicine , 55 (9). pp. 1434-6621. ISSN: 1437-4331 | doi: https://dx.doi.org/10.1515/cclm-2016-1054
- Castelletto, V. , Ryumin, P. , Cramer, R. , Hamley, I. , Taylor, M. , Allsop, D. , Reza, M. , Ruokolainen, J. , Arnold, T. , Hermida-Merino, D. , Garcia, C. , Leal, M. , Castaño, E. (2017) Self-assembly and anti-amyloid cytotoxicity activity of amyloid beta peptide derivatives. Scientific Reports , 7 ISSN: 2045-2322 | doi: https://dx.doi.org/10.1038/srep43637
- Ryumin, P. , Brown, J. , Morris, M. , Cramer, R. (2017) Protein identification using a nanoUHPLC-AP-MALDI MS/MS workflow with CID of multiply charged proteolytic peptides. International Journal of Mass Spectrometry , 416 pp. 20-28. ISSN: 1387-3806 | doi: https://dx.doi.org/10.1016/j.ijms.2016.12.006
- Wiangnon, K. and Cramer, R. (2016) Liquid MALDI MS analysis of complex peptide and proteome samples. Journal of Proteome Research , 15 (9). pp. 2998-3008. ISSN: 1535-3907 | doi: https://dx.doi.org/10.1021/acs.jproteome.6b00148
- Timms, J. , Hale, O. , Cramer, R. (2016) Advances in mass spectrometry-based cancer research and analysis: from cancer proteomics to clinical diagnostics. Expert Review of Proteomics , 13 (6). pp. 593-607. ISSN: 1478-9450 | doi: https://dx.doi.org/10.1080/14789450.2016.1182431
- Bryant, L. , Patole, C. , Cramer, R. (2016) Proteomic analysis of the medicinal plant Artemisia annua: data from leaf and trichome extracts. Data In Brief , 7 pp. 325-331. ISSN: 2352-3409 | doi: https://dx.doi.org/10.1016/j.dib.2016.02.038
- Pennington, H. , Gheorghe, D. , Damerum, A. , Pliego, C. , Spanu, P. , Cramer, R. , Bindschedler, L. (2016) Interactions between the powdery mildew effector BEC1054 and barley proteins identify candidate host targets. Journal of Proteome Research , 15 (3). pp. 826-839. ISSN: 1535-3907 | doi: https://dx.doi.org/10.1021/acs.jproteome.5b00732
- Ryumin, P. , Brown, J. , Morris, M. , Cramer, R. (2016) Investigation and optimization of parameters affecting the multiply charged ion yield in AP-MALDI MS. Methods , 104 pp. 11-20. ISSN: 1046-2023 | doi: https://dx.doi.org/10.1016/j.ymeth.2016.01.015
- Wiangnon, K. and Cramer, R. (2016) Coupling liquid MALDI MS to liquid chromatography. In: Cramer, R. , (eds.) Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry. Springer pp. 65-76. ISBN: 9783319048185 | doi: https://dx.doi.org/10.1007/978-3-319-04819-2_4
- Towers, M. and Cramer, R. (2016) Ionic liquids and other liquid matrices for sensitive MALDI MS analysis. In: Cramer, R. , (eds.) Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry. Springer pp. 51-64. ISBN: 9783319048185 | doi: https://dx.doi.org/10.1007/978-3-319-04819-2_3
- Cramer, R. (2016) Preface. In: Cramer, R. , (eds.) Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry. Springer ISBN: 9783319048185
- Ryumin, P. and Cramer, R. (2016) Efficient production of multiply charged MALDI ions. In: Cramer, R. , (eds.) Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry. Springer pp. 37-47. ISBN: 9783319048185 | doi: https://dx.doi.org/10.1007/978-3-319-04819-2_2
- Cramer, R. , eds. (2016) Advances in MALDI and laser-induced soft ionization mass spectrometry. Springer ISBN: 9783319048185
- Nouretdinov, I. , Devetyarov, D. , Vovk, V. , Burford, B. , Camuzeaux, S. , Gentry-Maharaj, A. , Tiss, A. , Smith, C. , Luo, Z. , Chervonenkis, A. , Hallett, R. , Waterfield, M. , Cramer, R. , Timms, J. , Jacobs, I. , Menon, U. , Gammerman, A. (2015) Multiprobabilistic prediction in early medical diagnoses. Annals of Mathematics and Artificial Intelligence , 74 (1-2). pp. 203-222. ISSN: 1573-7470 | doi: https://dx.doi.org/10.1007/s10472-013-9367-5
- Flatley, B. , Quaye, C. , Johnson, E. , Freeman, A. , Muneer, A. , Minhas, S. , Paterson, J. , Musa, F. , Malone, P. , Cramer, R. (2015) Distribution analysis of the putative cancer marker S100A4 across invasive squamous cell carcinoma penile tissue. EuPA Open Proteomics , 7 pp. 1-10. ISSN: 2212-9685 | doi: https://dx.doi.org/10.1016/j.euprot.2015.02.001
- Bryant, L. , Flatley, B. , Patole, C. , Brown, G. , Cramer, R. (2015) Proteomic analysis of Artemisia annua – towards elucidating the biosynthetic pathways of the antimalarial pro-drug artemisinin. BMC Plant Biology , 15 (1). ISSN: 1471-2229 | doi: https://dx.doi.org/10.1186/s12870-015-0565-7
- Wiangnon, K. and Cramer, R. (2015) Sample preparation: a crucial factor for the analytical performance of rationally designed MALDI matrices. Analytical Chemistry , 87 (3). pp. 1485-1488. ISSN: 0003-2700 | doi: https://dx.doi.org/10.1021/ac504412p
- Flatley, B. , Malone, P. , Cramer, R. (2014) MALDI mass spectrometry in prostate cancer biomarker discovery. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics , 1844 (5). pp. 940-949. ISSN: 1570-9639 | doi: https://dx.doi.org/10.1016/j.bbapap.2013.06.015
- Flatley, B. , Wilmott, K. , Malone, P. , Cramer, R. (2014) MALDI MS profiling of post-DRE urine samples highlights the potential of b-Microseminoprotein as a marker for prostatic diseases. The Prostate , 74 (1). pp. 103-111. ISSN: 1097-0045 | doi: https://dx.doi.org/10.1002/pros.22736
- Cramer, R. , Karas, M. , Jaskolla, T. (2013) Enhanced MALDI MS sensitivity by weak base additives and glycerol sample coating. Analytical Chemistry , 86 (1). pp. 744-751. ISSN: 0003-2700 | doi: https://dx.doi.org/10.1021/ac403228d
- Kovaleva, V. , Cramer, R. , Krynytskyy, H. , Gout, I. , Gout, R. (2013) Analysis of tyrosine phosphorylation and phosphotyrosine-binding proteins in germinating seeds from Scots pine. Plant Physiology and Biochemistry , 67 pp. 33-40. ISSN: 0981-9428 | doi: https://dx.doi.org/10.1016/j.plaphy.2013.02.008
- Agrawal, G. , Sarkar, A. , Righetti, P. , Pedreschi, R. , Carpentier, S. , Wang, T. , Barkla, B. , Kohli, A. , Ndimba, B. , Bykova, N. , Rampitsch, C. , Zolla, L. , Rafudeen, M. , Cramer, R. , Bindschedler, L. , Tsakirpaloglou, N. , Job, D. , Kikuchi, S. , Rakwal, R. (2013) A decade of plant proteomics and mass spectrometry: translation of technical advancements to food security and safety issues. Mass Spectrometry Reviews , 32 (5). pp. 335-365. ISSN: 1098-2787 | doi: https://dx.doi.org/10.1002/mas.21365
- Cramer, R. , Pirkl, A. , Hillenkamp, F. , Dreisewerd, K. (2013) AP-UV-MALDI mit flüssigen Matrizes: stabile Ionenausbeuten von mehrfach geladenen Peptid- und Proteinionen für die empfindliche Massenspektrometrie. Angewandte Chemie-International Edition , 125 (8). pp. 2420-2424. ISSN: 1521-3773 | doi: https://dx.doi.org/10.1002/ange.201208628
- Greenland, B. , Bird, M. , Burattini, S. , Cramer, R. , O'Reilly, R. , Patterson, J. , Hayes, W. , Cardin, C. , Colquhoun, H. (2013) Mutual binding of polymer end-groups by complementary π–π-stacking: a molecular “Roman Handshake”. Chemical Communications , 49 (5). pp. 454-456. ISSN: 1359-7345 | doi: https://dx.doi.org/10.1039/C2CC35965K
- Cramer, R. , Pirkl, A. , Hillenkamp, F. , Dreisewerd, K. (2013) Liquid AP-UV-MALDI enables stable ion yields of multiply charged peptide and protein ions for sensitive analysis by mass spectrometry. Angewandte Chemie-International Edition , 52 (8). pp. 2364-2367. ISSN: 1521-3773 | doi: https://dx.doi.org/10.1002/anie.201208628
- Cramer, R. and Westermeier, R. , eds. (2012) Difference Gel Electrophoresis (DIGE). Humana Press ISBN: 9781617795725 | doi: https://dx.doi.org/10.1007/978-1-61779-573-2
- Pedersen, C. , van Themaat, E. , McGuffin, L. , Abbott, J. , Burgis, T. , Barton, G. , Bindschedler, L. , Lu, X. , Maekawa, T. , Weßling, R. , Cramer, R. , Thordal-Christensen, H. , Panstruga, R. , Spanu, P. (2012) Structure and evolution of barley powdery mildew effector candidates. BMC Genomics , 13 (1). pp. 694. ISSN: 1471-2164 | doi: https://dx.doi.org/10.1186/1471-2164-13-694
- Smith, C. , Mills, D. , Cramer, R. (2012) Application of DIGE and mass spectrometry in the study of type 2 Diabetes Mellitus (T2DM) mouse models. In: Cramer, R. and Westermeier, R. , (eds.) Difference Gel Electrophoresis (DIGE). Methods in molecular biology Methods in Molecular Biology (854). pp. 299-318. ISSN: 1064-3745 ISBN: 9781617795725 | doi: https://dx.doi.org/10.1007/978-1-61779-573-2_21
- Sarsby, J. , Towers, M. , Stain, C. , Cramer, R. , Koroleva, O. (2012) Mass spectrometry imaging of glucosinolates in arabidopsis flowers and siliques. Phytochemistry , 77 pp. 110-118. ISSN: 0031-9422 | doi: https://dx.doi.org/10.1016/j.phytochem.2012.01.026
- Agrawal, G. , Sarkar, A. , Agrawal, R. , Ndimba, B. , Tanou, G. , Dunn, M. , Kieselbach, T. , Cramer, R. , Weinkoop, S. , Chen, S. , Rafudeen, M. , Deswal, R. , Barkla, B. , Weckwerth, W. , Heazlewood, J. , Renaut, J. , Job, D. , Chakraborty, N. , Rakwal, R. (2012) Boosting the globalization of plant proteomics through INPPO: current developments 3 and future prospects. Proteomics , 12 (3). pp. 359-368. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.201290018
- Cramer, R. (2011) Editorial for “Advances in Biological Mass Spectrometry and Proteomics”. Methods , 54 (4). pp. 349-350. ISSN: 1046-2023 | doi: https://dx.doi.org/10.1016/j.ymeth.2011.07.007
- Timms, J. , Menon, U. , Devetyarov, D. , Tiss, A. , Camuzeaux, S. , McCurrie, K. , Nouretdinov, I. , Burford, B. , Smith, C. , Gentry-Maharaj, A. , Hallett, R. , Ford, J. , Luo, Z. , Vovk, V. , Gammerman, A. , Cramer, R. , Jacobs, I. (2011) Early detection of ovarian cancer in samples pre-diagnosis using CA125 and MALDI-MS peaks. Cancer Genomics and Proteomics , 8 (6). pp. 289-305. ISSN: 1790-6245
- Koroleva, O. and Cramer, R. (2011) Single-cell proteomic analysis of glucosinolate-rich S-cells in Arabidopsis thaliana. Methods , 54 (4). pp. 413-423. ISSN: 1046-2023 | doi: https://dx.doi.org/10.1016/j.ymeth.2011.06.005
- Bauer, C. , Kleinjung, F. , Smith, C. , Towers, M. , Tiss, A. , Chadt, A. , Dreja, T. , Beule, D. , Al-Hasani, H. , Reinert, K. , Schuchhardt, J. , Cramer, R. (2011) Biomarker discovery and redundancy reduction towards classification using a multi-factorial MALDI-TOF MS T2DM mouse model dataset. BMC Bioinformatics , 12 ISSN: 1471-2105 | doi: https://dx.doi.org/10.1186/1471-2105-12-140
- Bindschedler, L. and Cramer, R. (2011) Fully automated software solution for protein quantitation by global metabolic labeling with stable isotopes. Rapid Communications in Mass Spectrometry , 25 (11). pp. 1461-1471. ISSN: 1097-0231 | doi: https://dx.doi.org/10.1002/rcm.4872
- Bindschedler, L. and Cramer, R. (2011) Quantitative plant proteomics. PROTEOMICS , 11 (4). pp. 756-775. ISSN: 1615-9861 | doi: https://dx.doi.org/10.1002/pmic.201000426
- Stringano, E. , Cramer, R. , Hayes, W. , Smith, C. , Gibson, T. , Mueller-Harvey, I. (2011) Deciphering the complexity of sainfoin (Onobrychis viciifolia) proanthocyanidins by MALDI-TOF mass spectrometry with a judicious choice of isotope patterns and matrixes. Analytical Chemistry , 83 (11). pp. 4147-4153. ISSN: 0003-2700 | doi: https://dx.doi.org/10.1021/ac2003856
- Bindschedler, L. , McGuffin, L. , Burgis, T. , Spanu, P. , Cramer, R. (2011) Proteogenomics and in silico structural and functional annotation of the barley powdery mildew Blumeria graminis f. sp. hordei. Methods , 54 (4). pp. 432-441. ISSN: 1046-2023 | doi: https://dx.doi.org/10.1016/j.ymeth.2011.03.006
- Bauer, C. , Cramer, R. , Schuchhardt, J. (2011) Evaluation of peak-picking algorithms for protein mass spectrometry. In: Hamacher, M. , Eisenacher, M. , Stephan, C. , (eds.) Data mining in proteomics: from standards to applications. Methods in molecular biology , 696. Humana Press , USA. pp. 341-352. ISBN: 9781607619864 | doi: https://dx.doi.org/10.1007/978-1-60761-987-1_22
- Tiss, A. , Smith, C. , Cramer, R. (2011) MALDI MS-based biomarker profiling of blood samples. In: Ivanov, A. and Lazarev, A. , (eds.) Sample preparation in biological mass spectrometry. Springer , New York. pp. 733-747. ISBN: 9789400707580
- Bindschedler, L. , Smith, C. , Cramer, R. (2011) Quantitative plant proteomics using hydroponic isotope labeling of entire plants (HILEP). In: Ivanov, A. and Lazarev, A. , (eds.) Sample preparation in biological mass spectrometry. , XVI. Springer , New York. ISBN: 9789400707580
- Stringano, E. , Hayot, C. , Smith, L. , Theodoridou, K. , Aufrere, J. , Brown, R. , Hayes, W. , Cramer, R. , Mueller-Harvey, I. (2010) Thiolytic screening method for exploring condensed tannin variation in a unique sainfoin germplasm bank. Planta Medica , 76 (12). ISSN: 1439-0221 | doi: https://dx.doi.org/10.1055/s-0030-1264189
- Stringano, E. , Gea, A. , Cramer, R. , Hayes, W. , Mueller-Harvey, I. (2010) Structural diversity of sainfoin (Onobrychis viciifolia) tannins.
- Tiss, A. , Timms, J. , Smith, C. , Devetyarov, D. , Gentry-Maharaj, A. , Camuzeaux, S. , Burford, B. , Nouretdinov, I. , Ford, J. , Luo, Z. , Jacobs, I. , Menon, U. , Gammerman, A. , Cramer, R. (2010) Highly accurate detection of ovarian cancer using CA125 but limited improvement with serum matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling. International Journal of Gynecological Cancer , 20 (9). pp. 1518-1524. ISSN: 1048-891x | doi: https://dx.doi.org/10.1111/IGC.0b013e3181fc1990
- Spanu, P. , Abbott, J. , Amselem, J. , Burgis, T. , Soanes, D. , Stuber, K. , Loren van Themaat, E. , Brown, J. , Butcher, S. , Gurr, S. , Lebrun, M. , Ridout, C. , Schulze-Lefert, P. , Talbot, N. , Ahmadinejad, N. , Ametz, C. , Barton, G. , Benjdia, M. , Bidzinski, P. , Bindschedler, L. , Both, M. , Brewer, M. , Cadle-Davidson, L. , Cadle-Davidson, M. , Collemare, J. , Cramer, R. , Frenkel, O. , Godfrey, D. , Harriman, J. , Hoede, C. , King, B. , Klages, S. , Kleemann, J. , Knoll, D. , Koti, P. , Kreplak, J. , Lopez-Ruiz, F. , Lu, X. , Maekawa, T. , Mahanil, S. , Micali, C. , Milgroom, M. , Montana, G. , Noir, S. , O'Connell, R. , Oberhaensli, S. , Parlange, F. , Pedersen, C. , Quesneville, H. , Reinhardt, R. , Rott, M. , Sacristan, S. , Schmidt, S. , Schon, M. , Skamnioti, P. , Sommer, H. , Stephens, A. , Takahara, H. , Thordal-Christensen, H. , Vigouroux, M. , Wessling, R. , Wicker, T. , Panstruga, R. (2010) Genome expansion and gene loss in powdery mildew fungi reveal tradeoffs in extreme parasitism. Science , 330 (6010). pp. 1543-1546. ISSN: 1095-9203 | doi: https://dx.doi.org/10.1126/science.1194573
- Flatley, B. , Malone, P. , Cramer, R. (2010) MS-based clinical proteomics: biomarker discovery in men’s cancer . European Pharmaceutical Review (5). pp. 53-56. ISSN: 1360-8606
- Timms, J. , Cramer, R. , Camuzeaux, S. , Tiss, A. , Smith, C. , Burford, B. , Nouretdinov, I. , Devetyarov, D. , Gentry-Maharaj, A. , Ford, J. , Luo, Z. , Gammerman, A. , Menon, U. , Jacobs, I. (2010) Peptides generated ex vivo from serum proteins by tumor-specific exopeptidases are not useful biomarkers in ovarian cancer. Clinical Chemistry , 56 (2). pp. 262-271. ISSN: 1530-8561 | doi: https://dx.doi.org/10.1373/clinchem.2009.133363
- Towers, M. , McKendrick, J. , Cramer, R. (2010) Introduction of 4-chloro-alpha-cyanocinnamic acid liquid matrices for high sensitivity UV-MALDI MS. Journal of Proteome Research , 9 (4). pp. 1931-1940. ISSN: 1535-3893 | doi: https://dx.doi.org/10.1021/pr901089j
- Fenton, T. , Gwalter, J. , Cramer, R. , Gout, I. (2010) S6K1 is acetylated at lysine 516 in response to growth factor stimulation. Biochemical and biophysical research communications , 398 (3). pp. 400-405. ISSN: 1090-2104 | doi: https://dx.doi.org/10.1016/j.bbrc.2010.06.081
- Koroleva, O. , Gibson, T. , Cramer, R. , Stain, C. (2010) Glucosinolate-accumulating S-cells in Arabidopsis leaves and flower stalks undergo programmed cell death at early stages of differentiation. The Plant Journal , 64 (3). pp. 456-469. ISSN: 0960-7412 | doi: https://dx.doi.org/10.1111/j.1365-313X.2010.04339.x
- Tiss, A. , Smith, C. , Menon, U. , Jacobs, I. , Timms, J. , Cramer, R. (2010) A well-characterised peak identification list of MALDI MS profile peaks for human blood serum. Proteomics , 10 (18). pp. 3388-3392. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.201000100
- Bindschedler, L. , Towers, M. , Cramer, R. (2009) Hydroponic isotope labeling of entire plants (HILEP) for quantitative plant proteomics.
- Bindschedler, L. , Burgis, T. , Mills, D. , Ho, J. , Spanu, P. , Cramer, R. (2009) In planta proteomics and proteogenomics of the biotrophic barley fungal pathogen blumeria f.sp. hordei.
- Ortiz-Chao, . , Gomez-Ruiz, . , Rastall, . , Mills, D. , Cramer, . , Pihlanto, . , Korhonen, . , Jauregi, . (2009) Production of novel ACE inhibitory peptides from beta-lactoglobulin using Protease N Amano . International Dairy Journal , 19 (2). pp. 69-76. ISSN: 0958-6946 | doi: https://dx.doi.org/10.1016/j.idairyj.2008.07.011
- Palmblad, . , Tiss, . , Cramer, . (2009) Mass spectrometry in clinical proteomics - from the present to the future. Proteomics Clinical Applications , 3 (1). pp. 6-17. ISSN: 1862-8346 | doi: https://dx.doi.org/10.1002/prca.200800090
- Kovaleva, . , Kiyamova, . , Cramer, . , Krynytskyy, . , Gout, . , Filonenko, . , Gout, . (2009) Purification and molecular cloning of antimicrobial peptides from Scots pine seedlings. Peptides , 30 (12). pp. 2136-2143. ISSN: 0196-9781 | doi: https://dx.doi.org/10.1016/j.peptides.2009.08.007
- Gammerman, . , Vovk, . , Burford, . , Nouretdinov, . , Luo, . , Chervonenkis, . , Waterfield, . , Cramer, . , Tempst, . , Villanueva, . , Kabir, . , Camuzeaux, . , Timms, . , Menon, . , Jacobs, . (2009) Serum proteomic abnormality predating screen detection of ovarian cancer. Computer Journal , 52 (3). pp. 326-333. ISSN: 0010-4620 | doi: https://dx.doi.org/10.1093/comjnl/bxn021
- Bindschedler, L. , Burgis, T. , Mills, D. , Ho, J. , Cramer, R. , Spanu, P. (2009) In planta proteomics and proteogenomics of the biotrophic barley fungal pathogen blumeria graminis f. sp hordei. Molecular & Cellular Proteomics , 8 (10). pp. 2368-2381. ISSN: 1535-9476 | doi: https://dx.doi.org/10.1074/mcp.M900188-MCP200
- Towers, . , Rollin, . , Tiss, . , Cramer, . (2009) Is liquid UV-MALDI a real alternative to solid state UV-MALDI?.
- Towers, . , McKendrick, J. , Cramer, . (2009) High Sensitivity α-cyano-4-Chlorocinnamic Acid Liquid Matrices for UV-MALDI MS.
- Tiss, . , Smith, . , Menon, . , Jacobs, . , Timms, . , Cramer, . (2009) An extensive peptide identification list of MALDI MS profile peaks from the analysis of human blood serum.
- Cramer, . (2009) MALDI MS. In: Reinders, . and Sickmann, . , (eds.) Proteomics: methods and protocols. Methods in molecular biology , 564. Humana Press , Totowa, New Jersey. pp. 85-103. ISBN: 9781607611561 | doi: https://dx.doi.org/10.1007/978-1-60761-157-8_5
- Timms, . and Cramer, . (2008) Difference gel electrophoresis. Proteomics , 8 (23-24). pp. 4886-4897. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200800298
- Huang, H. , Cendan, C. , Roza, C. , Okuse, K. , Cramer, R. , Timms, J. , Wood, J. (2008) Proteomic profiling of neuromas reveals alterations in protein composition and local protein synthesis in hyper-excitable nerves. Molecular Pain , 4 pp. 33. ISSN: 1744-8069 | doi: https://dx.doi.org/10.1186/1744-8069-4-33
- Bindschedler, . , Palmblad, . , Cramer, . (2008) Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study. Phytochemistry , 69 (10). pp. 1962-1972. ISSN: 0031-9422 | doi: https://dx.doi.org/10.1016/j.phytochem.2008.04.007
- Towers, . and Cramer, . (2008) High-sensitivity liquid UV-MALDI-MS analysis. pp. TP 047.
- Tiss, . , Smith, . , Timms, . , Luo, . , Gammerman, . , Waterfield, . , Menon, . , Jacobs, . , Cramer, . (2008) A robust and sensitive automated method for MS-based serum pattern diagnostics using well-established ZipTip technology and ultrafiltration. pp. MP 624.
- Bindschedler, . and Cramer, . (2008) Equalisation of protein amounts for overcoming the high dynamic protein expression range in the analysis of plant proteomes. pp. MP 573.
- Palmblad, M. , Towers, M. , Cramer, R. (2007) Multiplexed quantitative proteomics using differential metabolic 15N-labeling.
- Palmbland, M. , Towers, M. , Cramer, R. (2007) Investigation of liquid MALDI and optimization for instrument tuning and quantitative measurements.
- Cramer, R. and Dreisewerd, K. (2007) UV matrix-assisted laser desorption ionization: principles instrumentation and applications. In: Gross, M. and Capriolo, R. , (eds.) The encyclopedia of mass spectrometry. , 6. Elsevier ISBN: 9780080438016
- Tiss, A. , Smith, C. , Camuzeaux, S. , Kabir, M. , Gayther, S. , Menon, U. , Waterfield, M. , Timms, J. , Jacobs, I. , Cramer, R. (2007) Serum peptide profiling using MALDI mass spectrometry. Proteomics , 7 (S1). pp. 77-89. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200700746
- Towers, . and Cramer, . (2007) Liquid matrices for analyses by UV-MALDI mass spectrometry. Spectroscopy , 22 (11). pp. 29-. ISSN: 0887-6703
- Palmblad, . , Mills, D. , Bindschedler, . , Cramer, . (2007) Chromatographic alignment of LC-MS and LC-MS/MS datasets by genetic algorithm feature extraction. Journal of the American Society for Mass Spectrometry , 18 (10). pp. 1835-1843. ISSN: 1044-0305 | doi: https://dx.doi.org/10.1016/j.jasms.2007.07.018
- Palmblad, . , Bindschedler, . , Cramer, . (2007) Quantitative proteomics using uniform N-15-labeling, MASCOT, and the trans-proteomic pipeline. Proteomics , 7 (19). pp. 3462-3469. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200700180
- Palmblad, M. and Cramer, R. (2007) Liquid matrix deposition on conductive hydrophobic surfaces for tuning and quantitation in UV-MALDI mass spectrometry. Journal of the American Society of Mass Spectrometry , 18 (4). pp. 693-697. ISSN: 1044-0305 | doi: https://dx.doi.org/10.1016/j.jasms.2006.11.013
- Bindschedler, L. , Palmblad, M. , Cramer, R. (2006) First-dimension separation with the MicroRotoforTM cell prior to SDS-PAGE and LC-MS/MS analysis. Bio-Rad Bulletin , 5451 pp. 1-4.
- Palmblad, M. , Bindschedler, L. , Gibson, T. , Cramer, R. (2006) Comparison of bottom-up protein identification methods.
- Jacob, . and Cramer, . (2006) PIGOK: linking protein identity to gene ontology and function. Journal of Proteome Research , 5 (12). pp. 3429-3432. ISSN: 1535-3893 | doi: https://dx.doi.org/10.1021/pr0601537
- Palmblad, . , Bindschedler, . , Gibson, T. , Cramer, . (2006) Automatic internal calibration in liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry of protein digests. Rapid Communications in Mass Spectrometry , 20 (20). pp. 3076-3080. ISSN: 0951-4198 | doi: https://dx.doi.org/10.1002/rcm.2707
- Nagano, . , Bornhauser, . , Warnasuriya, . , Entwistle, . , Cramer, . , Lindholm, . , Naaby-Hansen, . (2006) PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E-3-ligase MIR. EMBO Journal , 25 (9). pp. 1871-1882. ISSN: 0261-4189 | doi: https://dx.doi.org/10.1038/sj.emboj.7601059
- Jacob, R. , Thompson, A. , Cramer, R. (2005) A preliminary comparative analysis of the H. sapiens saliva proteome between cysteine fractionation,performic acid oxidation LC/MALDI/MS/MS and LC/ESI/MS/MS.
- Weeks, . , Sinclair, . , Jacob, . , Saxton, . , Kirby, . , Jones, . , Waterfield, . , Cramer, . , Timms, . (2005) Stress-induced changes in the Schizosaccharomyces pombe proteome using two-dimensional difference gel electrophoresis, mass spectrometry and a novel integrated robotics platform. Proteomics , 5 (6). pp. 1669-1685. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200401241
- Hastie, . , Saxton, . , Akpan, . , Cramer, . , Masters, . , Naaby-Hans, . (2005) Combined affinity labelling and mass spectrometry analysis of differential cell surface protein expression in normal and prostate cancer cells. Oncogene , 24 (38). pp. 5905-5913. ISSN: 0950-9232 | doi: https://dx.doi.org/10.1038/sj.onc.1208747
- de Graauw, . , Tijdens, . , Cramer, . , Corless, . , Timms, . , van de Water, . (2005) Heat shock protein 27 is the major differentially phosphorylated protein involved in renal epithelial cellular stress response and controls focal adhesion organization and apoptosis. The Journal of Biological Chemistry , 280 (33). pp. 29885-29898. ISSN: 1083-351X | doi: https://dx.doi.org/10.1074/jbc.M412708200
- Cutillas, . , Biber, . , Marks, . , Jacob, . , Stieger, . , Cramer, . , Waterfield, . , Burlingame, . , Unwin, . (2005) Proteomic analysis of plasma membrane vesicles isolated from the rat renal cortex. Proteomics , 5 (1). pp. 101-112. ISSN: 1615-9853
- Cramer, . , Gobom, . , Nordhoff, . (2005) High-throughput proteomics using matrix-assisted laser desorption/ionization mass spectrometry. Expert Review of Proteomics , 2 (3). pp. 407-420. ISSN: 1478-9450 | doi: https://dx.doi.org/10.1586/14789450.2.3.407
- Cramer, . and Corless, . (2005) Liquid ultraviolet matrix-assisted laser desorption/ionization mass spectrometry for automated proteomic analysis. Proteomics , 5 (2). pp. 360-370. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200400956
- Chan, . , Gharbi, . , Gaffney, . , Cramer, . , Waterfield, . , Timms, . (2005) Proteomic analysis of redox- and ErbB2-dependent changes in mammary luminal epithelial cells using cysteine- and lysine-labelling two-dimensional difference gel electrophoresis. Proteomics , 5 (11). pp. 2908-2926. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200401300
- Barnouin, . , Hart, . , Thompson, . , Okuyama, . , Waterfield, . , Cramer, . (2005) Enhanced phosphopeptide isolation by Fe(III)-IMAC using 1,1,1,3,3,3-hexafluoroisopropanol. Proteomics , 5 (17). pp. 4376-4388. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200401323
- Naaby-Hansen, . , Warnasuriya, . , Hastie, . , Gallney, . , Cramer, . (2005) Proteomic approaches in the analysis of hypertension. In: Fennell, . and Baker, . , (eds.) Hypertension: methods and protocols . Methods in molecular medicine , 108. Humana Press , New Jersey. pp. 275-296. ISBN: 9781588293237