Becky Green
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+44 (0) 118 378 8446
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Professor of Biophysical Chemistry
- School Director of Recruitment and Admissions (School of Chemistry, Food and Pharmacy)
- Admissions Tutor and Programme Director for MPAS Physician Associate Studies
Areas of interest
Rebecca's scientific interests lie in the study of protein behaviour, and in linking their structural features to functional properties. Most recently, her research has focused on the lipid (cell membrane) binding behaviour of antimicrobial and anticancer peptides and proteins.
Within the area of food security, Rebecca's recent research has focused on seed defence proteins and peptides, particularly the functional properties of puroindolines and purothionins. These proteins also have potential, with antifungal and antimicrobial activity, pharmaceutically.
From the point of view of lipid biophysics, her research has focused on defining the role of different lipid constituents within biological membranes and how these provide potential binding sites for polymers, peptides and proteins.
Rebecca also has broader research interests in the areas of biomaterials and biophysical chemistry, and works on a number of related projects, including protein-polyphenol interactions, formulation solutions for poorly-water soluble drugs and polymer binding to lipid surfaces in advanced drug delivery. Her research regularly utilises STFC and other central facilities; neutron (ISIS, ILL) and X-ray (Diamond) reflectivity and scattering methods.
Publications
- Virtanen, V. , Green, R. , Karonen, M. (2022) Interactions between Hydrolysable Tannins and Lipid Vesicles from Escherichia coli with Isothermal Titration Calorimetry. Molecules , 27 (10). ISSN: 1420-3049 | doi: https://dx.doi.org/10.3390/molecules27103204
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Coones, R.
ORCID: 0000-0001-6651-4707
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Green, R.
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Frazier, R.
(2021)
Investigating lipid headgroup composition within epithelial membranes: a systematic review.
Soft Matter
ISSN:
1744-6848
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doi:
https://dx.doi.org/10.1039/D1SM00703C
- Karonen, M. , Oraviita, M. , Mueller-Harvey, I. , Salminen, J. , Green, R. (2019) Ellagitannins with a glucopyranose core have higher affinity to proteins than acyclic ellagitannins by isothermal titration calorimetry. Journal of Agricultural and Food Chemistry , 67 (46). pp. 12730-12740. ISSN: 0021-8561 | doi: https://dx.doi.org/10.1021/acs.jafc.9b04353
- English, M. , Paulson, A. , Green, R. , Florek, O. , Clifton, L. , Arnold, T. , Frazier, R. (2019) The effects of native and modified clupeine on the structure of gram-negative model membranes. Food Structure , 22 ISSN: 2213-3291 | doi: https://dx.doi.org/10.1016/j.foostr.2019.100127
- Florek, O. , Clifton, L. , Wilde, M. , Arnold, T. , Green, B. , Frazier, R. (2018) Lipid composition in fungal membrane models: effect of lipid fluidity. Acta Crystallographica Section D: Biological Crystallography , 74 (12). pp. 1233-1244. ISSN: 1399-0047 | doi: https://dx.doi.org/10.1107/S2059798318009440
- Wilde, M. , Green, R. , Sanders, M. , Greco, F. (2017) Biophysical studies in polymer therapeutics: the interactions of anionic and cationic PAMAM dendrimers with lipid monolayers. Journal of Drug Targeting , 25 (9-10). pp. 910-918. ISSN: 1029-2330 | doi: https://dx.doi.org/10.1080/1061186X.2017.1365877
- Sanders, M. , Clifton, L. , Frazier, R. , Green, R. (2017) Tryptophan to arginine substitution in puroindoline b alters binding to model eukaryotic membranes. Langmuir , 33 (19). pp. 4847-4853. ISSN: 0743-7463 | doi: https://dx.doi.org/10.1021/acs.langmuir.6b03030
- Ropiak, H. , Lachmann, P. , Ramsay, A. , Green, R. , Mueller-Harvey, I. (2017) Identification of structural features of condensed tannins that affect protein aggregation. PLoS ONE , 12 (1). ISSN: 1932-6203 | doi: https://dx.doi.org/10.1371/journal.pone.0170768
- Sanders, M. , Clifton, L. , Frazier, R. , Green, R. (2016) The role of lipid composition on the interaction between a tryptophan-rich protein and model bacterial membranes. Langmuir , 32 (8). pp. 2050-2057. ISSN: 0743-7463 | doi: https://dx.doi.org/10.1021/acs.langmuir.5b04628
- Karonen, M. , Oraviita, M. , Mueller-Harvey, I. , Salminen, J. , Green, R. (2015) Binding of an oligomeric ellagitannin series to bovine serum albumin (BSA): analysis by isothermal titration calorimetry (ITC). Journal of Agricultural and Food Chemistry , 63 (49). pp. 10647-10654. ISSN: 0021-8561 | doi: https://dx.doi.org/10.1021/acs.jafc.5b04843
- Albarkah, Y. , Green, R. , Khutoryanskiy, V. (2015) Probing the mucoadhesive interactions between porcine gastric mucin and some water-soluble polymers. Macromolecular Bioscience , 15 (11). pp. 1546-1553. ISSN: 1616-5187 | doi: https://dx.doi.org/10.1002/mabi.201500158
- Ravishankar, D. , Watson, K. , Boateng, S. , Green, R. , Greco, F. , Osborn, H. (2015) Exploring quercetin and luteolin derivatives as antiangiogenic agents. European Journal of Medicinal Chemistry , 97 pp. 259-274. ISSN: 0223-5234 | doi: https://dx.doi.org/10.1016/j.ejmech.2015.04.056
- Dabkowska, A. , Barlow, D. , Clifton, L. , Hughes, A. , Webster, J. , Green, R. , Quinn, P. , Lawrence, M. (2014) Calcium-mediated binding of DNA to 1,2-distearoyl-sn-glycero-3-phosphocholine-containing mixed lipid monolayers. Soft Matter , 10 (11). pp. 1685-1695. ISSN: 1744-683X | doi: https://dx.doi.org/10.1039/c3sm52747f
- Dobreva, M. , Green, R. , Mueller-Harvey, I. , Salminen, J. , Howlin, B. , Frazier, R. (2014) Size and molecular flexibility affect the binding of ellagitannins to bovine serum albumin. Journal of Agricultural and Food Chemistry , 62 (37). pp. 9186-9194. ISSN: 0021-8561 | doi: https://dx.doi.org/10.1021/jf502174r
- Sanders, M. , Clifton, L. , Neylon, C. , Frazier, R. , Green, R. (2013) Selected wheat seed defense proteins exhibit competitive binding to model microbial lipid interfaces. Journal of Agricultural and Food Chemistry , 61 (28). pp. 6890-6900. ISSN: 1520-5118 | doi: https://dx.doi.org/10.1021/jf401336a
- Clifton, L. , Sanders, M. , Kinane, C. , Arnold, T. , Edler, K. , Neylon, C. , Green, R. , Frazier, R. (2012) The role of protein hydrophobicity in thionin–phospholipid interactions: a comparison of α1 and α2-purothionin adsorbed anionic phospholipid monolayers. Physical Chemistry Chemical Physics , 14 (39). pp. 13569-13579. ISSN: 1463-9076 | doi: https://dx.doi.org/10.1039/C2CP42029E
- Clifton, L. , Sanders, M. , Hughes, A. , Neylon, C. , Frazier, R. , Green, R. (2011) Lipid binding interactions of antimicrobial plant seed defence proteins: puroindoline-a and β-purothionin. Physical Chemistry Chemical Physics , 13 (38). pp. 17153-17162. ISSN: 1463-9076 | doi: https://dx.doi.org/10.1039/c1cp21799b
- Rizi, K. , Green, R. , Donaldson, M. , Williams, A. (2011) Using pH abnormalities in diseased skin to trigger and target topical therapy. Pharmaceutical Research , 28 (10). pp. 2589-2598. ISSN: 0724-8741 | doi: https://dx.doi.org/10.1007/s11095-011-0488-4
- Rizi, K. , Green, B. , Donaldson, M. , Williams, A. (2011) Production of pH-responsive microparticles by spray drying: investigation of experimental parameter effects on morphological and release properties. Journal of Pharmaceutical Sciences , 100 (2). pp. 566-579. ISSN: 1520-6017 | doi: https://dx.doi.org/10.1002/jps.22291
- Rizi, K. , Green, R. , Khutoryanskaya, O. , Donaldson, M. , Williams, A. (2011) Mechanisms of burst release from pH-responsive polymeric microparticles.. Journal of Pharmacy and Pharmacology , 63 (9). pp. 1141-1155. ISSN: 0022-3573 | doi: https://dx.doi.org/10.1111/j.2042-7158.2011.01322.x
- Clifton, L. , Sanders, M. , Castelletto, V. , Rogers, S. , Heenan, R. , Neylon, C. , Frazier, R. , Green, R. (2011) Puroindoline-a, a lipid binding protein from common wheat, spontaneously forms prolate protein micelles in solution. Physical Chemistry Chemical Physics , 13 (19). pp. 8881-8888. ISSN: 1463-9076 | doi: https://dx.doi.org/10.1039/c0cp02247k
- Dobreva, M. , Frazier, R. , Mueller-Harvey, I. , Clifton, L. , Gea, A. , Green, R. (2011) Binding of pentagalloyl glucose to two globular proteins occurs via multiple surface sites. Biomacromolecules , 12 (3). pp. 710-715. ISSN: 1525-7797 | doi: https://dx.doi.org/10.1021/bm101341s
- Lambourne, J. , Tosi, P. , Marsh, J. , Bhandari, D. , Green, R. , Frazier, R. , Shewry, P. (2010) Characterisation of an s-type low molecular weight glutenin subunit of wheat and its proline and glutamine-rich repetitive domain. Journal of Cereal Science , 51 (1). pp. 96-104. ISSN: 0733-5210 | doi: https://dx.doi.org/10.1016/j.jcs.2009.10.003
- Frazier, R. , Deaville, E. , Green, R. , Stringano, E. , Willoughby, I. , Plant, J. , Mueller-Harvey, I. (2010) Interactions of tea tannins and condensed tannins with proteins. Journal of Pharmaceutical and Biomedical Analysis , 51 (2). pp. 490-495. ISSN: 0731-7085 | doi: https://dx.doi.org/10.1016/j.jpba.2009.05.035
- Deepika, G. , Green, R. , Frazier, R. , Charalampopoulos, D. (2009) Effect of growth time on the surface and adhesion properties of Lactobacillus rhamnosus GG. Journal of Applied Microbiology , 107 (4). pp. 1230-1240. ISSN: 1364-5072 | doi: https://dx.doi.org/10.1111/j.1365-2672.2009.04306.x
- Pasut, G. , Greco, F. , Mero, A. , Mendichi, R. , Fante, C. , Green, R. , Veronese, F. (2009) Polymer-drug conjugates for combination anticancer therapy: investigating the mechanism of action.. Journal of Medicinal Chemistry , 52 (20). pp. 6499-6502. ISSN: 0022-2623 | doi: https://dx.doi.org/10.1021/jm900804m
- Castelletto, . , Hamley, . , Clifton, . , Green, . (2008) Osmotic pressure and aggregate shape in BSA/poly (ethylene glycol)-lipid/Dextran solutions. Biophysical Chemistry , 134 (1-2). pp. 34-38. ISSN: 0301-4622 | doi: https://dx.doi.org/10.1016/j.bpc.2008.01.004
- Clifton, L. , Green, R. , Hughes, A. , Frazier, R. (2008) Interfacial structure of wild-type and mutant forms of puroindoline-b bound to DPPG monolayers. Journal of Physical Chemistry B , 112 (49). pp. 15907-15913. ISSN: 1520-6106 | doi: https://dx.doi.org/10.1021/jp806016h
- Pellenc, D. , Bennett, R. , Green, R. , Sperrin, M. , Mulheran, P. (2008) New insights on growth mechanisms of protein clusters at surfaces: an AFM and simulation study. Langmuir , 24 (17). pp. 9648-9655. ISSN: 0743-7463 | doi: https://dx.doi.org/10.1021/la801246k
- Mulheran, P. , Pellenc, D. , Bennett, R. , Green, R. , Sperrin, M. (2008) Mechanisms and dynamics of protein clustering on a solid surface. Physical Review Letters , 100 (6). ISSN: 0031-9007 | doi: https://dx.doi.org/10.1103/PhysRevLett.100.068102
- Clifton, . , Green, . , Frazier, . (2007) Lipid selectivity of puroindolines and its relationship to endosperm hardness. In: Lookhart, . and Ng, . , (eds.) Gluten proteins 2006. AACC International , St Paul. pp. 68-72. ISBN: 9781891127571
- Lad, . , Birembaut, . , Clifton, . , Frazier, . , Webster, . , Green, . (2007) Antimicrobial peptide-lipid binding interactions and binding selectivity. Biophysical Journal , 92 (10). pp. 3575-3586. ISSN: 0006-3495 | doi: https://dx.doi.org/10.1529/biophysj.106.097774
- Deaville, E. , Green, B. , Mueller-Harvey, I. , Willoughby, I. , Frazier, R. (2007) Hydrolyzable tannin structures influence relative globular and random coil protein binding strengths. Journal of Agricultural and Food Chemistry , 55 (11). pp. 4554-4561. ISSN: 0021-8561 | doi: https://dx.doi.org/10.1021/jf063770o
- Clifton, L. , Lad, M. , Green, R. , Frazier, R. (2007) Single amino acid substitutions in puroindoline-b mutants influence lipid binding properties. Biochemistry , 46 (8). pp. 2260-2266. ISSN: 0006-2960 | doi: https://dx.doi.org/10.1021/bi062190h
- Clifton, L. , Green, R. , Frazier, R. (2007) Puroindoline-b mutations control the lipid binding interactions in mixed puroindoline-a: puroindoline-b systems. Biochemistry , 46 (48). pp. 13929-13937. ISSN: 0006-2960 | doi: https://dx.doi.org/10.1021/bi701680w
- Frazier, R. , Papadopoulou, A. , Green, R. (2006) Isothermal titration calorimetry study of epicatechin binding to serum albumin. Journal of Pharmaceutical and Biomedical Analysis , 41 (5). pp. 1602-1605. ISSN: 0731-7085 | doi: https://dx.doi.org/10.1016/j.jpba.2006.02.004
- Lad, M. , Birembaut, F. , Matthew, J. , Frazier, R. , Green, R. (2006) The adsorbed conformation of globular proteins at the air/water interface. Physical Chemistry Chemical Physics , 8 (18). pp. 2179-2186. ISSN: 1463-9076 | doi: https://dx.doi.org/10.1039/b515934b
- Cooper, . , Kennedy, . , Fleming, . , Wokosin, . , Su, . , Green, . , Lu, . , Wilson, . , Videler, . (2005) Adsorption of frog foam nest proteins at the air-water interface. Biophysical Journal , 88 (3). pp. 2114-2125. ISSN: 0006-3495 | doi: https://dx.doi.org/10.1529/biophysj.104.046268
- Lad, . , Birembaut, . , Frazier, . , Green, . (2005) Protein-lipid interactions at the air/water interface. Physical Chemistry Chemical Physics , 7 (19). pp. 3478-3485. ISSN: 1463-9076 | doi: https://dx.doi.org/10.1039/b506558p
- Papadopoulou, A. , Green, R. , Frazier, R. (2005) Interaction of flavonoids with bovine serum albumin: a fluorescence quenching study . Journal of Agricultural and Food Chemistry , 53 (1). pp. 158-163. ISSN: 0021-8561 | doi: https://dx.doi.org/10.1021/jf048693g
- Jeganathan, P. , Green, R. , Norris, K. , Vogiatzakis, I. , Bartsch, A. , Wotton, S. , Bowden, C. , Griffiths, G. , Pain, D. , Rahmani, A. (2004) Modelling habitat selection and distribution of the critically endangered Jerdon's courser Rhinoptilus bitorquatus in scrub jungle: an application of a new tracking method. Journal of Applied Ecology , 41 (2). pp. 224-237. ISSN: 0021-8901 | doi: https://dx.doi.org/10.1111/j.0021-8901.2004.00897.x
- Frazier, . , Papadopoulou, . , Mueller-Harvey, . , Kissoon, . , Green, . (2003) Probing protein-tannin interactions by isothermal titration microcalorimetry. Journal of Agricultural and Food Chemistry , 51 (18). pp. 5189-5195. ISSN: 0021-8561 | doi: https://dx.doi.org/10.1021/jf021179v
- Lad, . , Ledger, . , Briggs, . , Green, . , Frazier, . (2003) Analysis of the SDS-lysozyme binding isotherm. Langmuir , 19 (12). pp. 5098-5103. ISSN: 0743-7463 | doi: https://dx.doi.org/10.1021/la0269560
- Lad, M. , Ledger, V. , Briggs, B. , Green, R. , Frazier, R. (2003) Analysis of the SDS−Lysozyme binding isotherm. Langmuir , 19 (12). pp. 5098-5103. ISSN: 0743-7463 | doi: https://dx.doi.org/10.1021/la0269560
- Frazier, R. , Papadopoulou, A. , Mueller-Harvey, I. , Kissoon, D. , Green, R. (2003) Probing protein−tannin interactions by isothermal titration microcalorimetry. Journal of Agricultural and Food Chemistry , 51 (18). pp. 5189-5195. ISSN: 0021-8561 | doi: https://dx.doi.org/10.1021/jf021179v