Darius Widera

Email:
d.widera@reading.ac.uk
Phone:
+44 (0) 118 378 7053
Job title:
Professor of Stem Cell Biology and Regenerative Medicine

Responsibilities:

Pharmacy Research Lead
Industrial Placement Lead (Pharmacology programme)
ERASMUS and Study Abroad Coordinator

Areas of interest

Mesenchymal Stromal/Stem Cells
Neural Crest-Derived Stem Cells
Neural Stem Cells
Cancer Stem Cells
3D cell culture
Extracellular vesicle biology
Toll-like Receptor 4 Signalling in Health and Disease
NF-kappaB Signalling

Background

Darius Widera is a Professor of Stem Cell Biology and Regenerative Medicine and Research Division Lead at the University of Reading. His lab is mainly interested in adult stem cells and their secretome, inflammatory signalling cascades in health and disease, and novel methods for clinical-grade 3D cultivation of human stem cells. His research is focused on the regenerative potential of mesenchymal stromal cells, neural crest-derived stem cells, and pluripotent stem cells in different disorders and conditions. Lastly, his research aims to tackle disorders and conditions with an inflammatory component, such as osteoporosis, lung, brain, and breast cancer.

He graduated in Biochemistry (Witten/Herdecke University, Germany) and received his PhD in Neurobiochemistry from Witten/Herdecke University, Germany, in 2007. From 2013 to 2015, he served as an Adjunct Professor at the Department of Cell Biology, University of Bielefeld (Germany). In February 2015, he was appointed as a Lecturer in Stem Cell Biology and Regenerative Medicine at the University of Reading, promoted to Associate Professor in 2019, and to full Professor in 2023. Since 2022, Prof. Widera has also been the Research Division Lead for the Reading School of Pharmacy at the UoR.

He has published over 90 research manuscripts (h-index of >32, i-10 index of >63) and has presented his research outcomes as invited talks at numerous international conferences in the UK, Germany, USA, Italy, Ireland, Finland, San Marino, Russia, Malaysia, Singapore, Brazil, and China. To date, he has supervised 11 PhD and 26 Master's students to completion. In addition to his academic duties, he has acted as a consultant for UPM Biomedicals and is currently a Scientific Advisory Board Member for Argon Medical Ltd, Germany.

Prof. Widera is also actively engaging in science communication, and he has consulted national and international media, including The Times, BBC, Daily Mail, AFP, Nature, and ITN.

External Roles:

Academic Editor, Stem Cells International
Associate Editor, Frontiers in Stem Cell Research (Section of Frontiers in Genetics)
Scientific Advisory Board Member, Argon Llt, Germany
External Examiner for the MRes Biomedical Sciences and Translational Medicine programme, University of Liverpool

Websites/blogs

Lab Webpage

Publications

Skurikhin, E. , Pershina, O. ORCID: 0000-0002-0468-0272 , Zhukova, M. ORCID: 0000-0002-8146-2279 , Pakhomova, A. ORCID: 0000-0003-0725-1323 , Ermakova, N. , Widera, D. ORCID: 0000-0003-1686-130X , Pan, E. ORCID: 0000-0002-2163-7647 , Sandrikina, L. , Kogai, L. , Kushlinskii, N. , Morozov, S. , Kubatiev, A. , Dygai, A. (2023) Reprogrammed CD8+ T-cells isolated from the mouse spleen increase the number of immune cells with antitumor activity and decrease the amount of cancer stem cells. Medical Science Forum , 21 (1). pp 0. ISSN: 2673-9992 | doi: https://dx.doi.org/10.3390/ecb2023-14132
Fareez, I. , Liew, F. , Widera, D. ORCID: 0000-0003-1686-130X , Mayeen, N. , Mawya, J. , Abu Kasim, N. , Haque, N. (2023) Application of platelet-rich plasma as a stem cell treatment - an attempt to clarify a common public misconception. Current Molecular Medicine , 23 pp 0. ISSN: 1875-5666 | doi: https://dx.doi.org/10.2174/1566524023666230511152646
Palaniyappan, K. , S. M. N. Mydin, R. ORCID: 0000-0001-8971-8809 , Widera, D. ORCID: 0000-0003-1686-130X , Noordin, S. , Harun, N. , Wan Eddis Effendy, W. , Hazan, R. , Sreekantan, S. (2023) Double-edged sword of biofouling potentials associated with haemocompatibility behaviour: titania nanotube arrays for medical implant surface technology. Beni-Suef University Journal of Basic and Applied Sciences , 12 (1). ISSN: 2314-8543 | doi: https://dx.doi.org/10.1186/s43088-023-00363-y
Asadpour, A. , Yahaya, B. , Bicknell, K. , Cottrell, G. ORCID: 0000-0001-9098-7627 , Widera, D. ORCID: 0000-0003-1686-130X (2023) Uncovering the gray zone: mapping the global landscape of direct-to-consumer businesses offering interventions based on secretomes, extracellular vesicles, and exosomes. Stem cell research and therapy , 14 (1). ISSN: 1757-6512 | doi: https://dx.doi.org/10.1186/s13287-023-03335-2

Skurikhin, E. , Pershina, O. , Ermakova, N. , Pakhomova, A. , Zhukova, M. , Pan, E. , Sandrikina, L. , Widera, D. ORCID: 0000-0003-1686-130X , Kogai, L. , Kushlinskii, N. , Kubatiev, A. , Morozov, S. , Dygai, A. (2022) Cell therapy with human reprogrammed CD8+ T-cells has antimetastatic effects on Lewis Lung Carcinoma in C57BL/6 mice. International Journal of Molecular Sciences , 23 (24). ISSN: 1422-0067 | doi: https://dx.doi.org/10.3390/ijms232415780
Skurikhin, E. , Ermakova, N. , Zhukova, M. ORCID: 0000-0002-8146-2279 , Pershina, O. ORCID: 0000-0002-0468-0272 , Pan, E. ORCID: 0000-0002-2163-7647 , Pakhomova, A. ORCID: 0000-0003-0725-1323 , Kogai, L. , Goldberg, V. , Simolina, E. , Skurikhina, V. , Widera, D. ORCID: 0000-0003-1686-130X , Kubatiev, A. , Morozov, S. , Kushlinskii, N. , Dygai, A. (2022) Analysis of circulating tumor and cancer stem cells provides new opportunities in diagnosis and treatment of small cell lung cancer. International Journal of Molecular Sciences , 23 (18). ISSN: 1422-0067 | doi: https://dx.doi.org/10.3390/ijms231810853
Skurikhin, E. , Ermakova, N. , Zhukova, M. , Pershina, O. , Pan, E. , Pakhomova, A. , Kogai, L. , Goldberg, V. , Simolina, E. , Skurikhina, V. , Widera, D. ORCID: 0000-0003-1686-130X , Kubatiev, A. , Morozov, S. , Kushlinskii, N. , Dygai, A. (2022) Analysis of circulating tumour and cancer stem cells provides new opportunities in diagnosis and treatment of small cell lung cancer. International Journal of Molecular Sciences , 23 (18). ISSN: 1422-0067 | doi: https://dx.doi.org/10.3390/ijms231810853
Gramignoli, R. , Sallustio, F. , Widera, D. ORCID: 0000-0003-1686-130X , eds. (2022) Tissue repair and regenerative mechanisms by stem/progenitor cells and their secretome, volume II. Frontiers in Medicine Frontiers | doi: https://dx.doi.org/10.3389/fmed.2022.901003
Kanapeckaitė, A. , Mažeikienė, A. , Geris, L. , Burokienė, N. , Cottrell, G. ORCID: 0000-0001-9098-7627 , Widera, D. ORCID: 0000-0003-1686-130X (2022) Computational pharmacology: new avenues for COVID-19 therapeutics search and better preparedness for future pandemic crises. Biophysical Chemistry , 290 ISSN: 0301-4622 | doi: https://dx.doi.org/10.1016/j.bpc.2022.106891
Skurikhin, E. , Pershina, O. ORCID: 0000-0002-0468-0272 , Ermakova, N. , Pakhomova, A. ORCID: 0000-0003-0725-1323 , Widera, D. ORCID: 0000-0003-1686-130X , Zhukova, M. ORCID: 0000-0002-8146-2279 , Pan, E. ORCID: 0000-0002-2163-7647 , Sandrikina, L. , Kogai, L. , Kushlinskii, N. , Morozov, S. , Kubatiev, A. , Dygai, A. (2022) Reprogrammed CD8+ T-lymphocytes isolated from bone marrow have anticancer potential in lung cancer. Biomedicines , 10 (6). ISSN: 2227-9059 | doi: https://dx.doi.org/10.3390/biomedicines10061450
Ridzuan, N. , Widera, D. ORCID: 0000-0003-1686-130X , Yahaya, B. (2022) Isolation and characterization of extracellular vesicles derived from human umbilical cord mesenchymal stem cells. In: Kannan, N. and Beer, P. , (eds.) Stem Cell Assays. Methods in Molecular Biology , 2429. Methods in Molecular Biology , 2429 (2429). pp. 271-280. ISSN: 1064-3745 ISBN: 9781071619797 | doi: https://dx.doi.org/10.1007/978-1-0716-1979-7_18 (ISSN 1940-6029)
Mydin, R. , Sreekantan, S. , Widera, D. ORCID: 0000-0003-1686-130X , Saharudin, K. , Hazan, R. , Wajidi, M. (2022) Genome-nanosurface interaction of Titania Nanotube Arrays: evaluation of telomere, telomerase and NF-κB activities on epithelial cell model. RSC Advances , 12 (4). pp. 2237-2245. ISSN: 2046-2069 | doi: https://dx.doi.org/10.1039/D1RA05325F
Haque, N. , Widera, D. ORCID: 0000-0003-1686-130X , Govindasamy, V. , Soesilawati, P. , Kasim, N. (2022) Extracellular vesicles from stem and progenitor cells for cell-free regenerative therapy. Current Molecular Medicine , 22 (2). pp. 120-131. ISSN: 1566-5240 | doi: https://dx.doi.org/10.2174/1566524021666210125114828

Skurikhin, E. , Pershina, O. , Zhukova, M. , Widera, D. ORCID: 0000-0003-1686-130X , Pan, E. , Pakhomova, A. , Krupin, V. , Ermakova, N. , Skurikhina, V. , Sandrikina, L. , Morozov, S. , Kubatiev, A. , Dygai, A. (2021) Spiperone stimulates regeneration in pulmonary endothelium damaged by cigarette smoke and lipopolysaccharide. International Journal of Chronic Obstructive Pulmonary Disease , 16 pp. 3575-3591. ISSN: 1178-2005 | doi: https://dx.doi.org/10.2147/COPD.S336410
Skurikhin, E. , Pershina, O. , Zhukova, M. , Widera, D. ORCID: 0000-0003-1686-130X , Ermakova, N. , Pan, E. , Pakhomova, A. , Morozov, S. , Kubatiev, A. , Dygai, A. (2021) Potential of stem cells and CART as a potential polytherapy for small cell lung cancer. Frontiers in Cell and Developmental Biology , 9 ISSN: 2296-634X | doi: https://dx.doi.org/10.3389/fcell.2021.778020
Widera, D. ORCID: 0000-0003-1686-130X , Ong, W. , Sheard, J. , eds. (2021) Recent advances in Translational Adipose-Derived Stem Cell Biology. , 11. Biomolecules , 11 (11). ISSN: 2218-273X | doi: https://dx.doi.org/10.3390/biom11111660
Alatawi, K. , Ravishankar, D. , Patra, P. , Bye, A. , Stainer, A. , Patel, K. , Widera, D. ORCID: 0000-0003-1686-130X , Vaiyapuri, S. ORCID: 0000-0002-6006-6517 (2021) 1,8-cineole affects agonists-induced platelet activation, thrombus formation and haemostasis. Cells , 10 (10). ISSN: 2073-4409 | doi: https://dx.doi.org/10.3390/cells10102616
Kanapeckaitė, A. , Burokienė, N. , Mažeikienė, A. , Cottrell, G. ORCID: 0000-0001-9098-7627 , Widera, D. ORCID: 0000-0003-1686-130X (2021) Biophysics is reshaping our perception of the epigenome: from changing the landscape of how we study DNA-level epigenetic marks to enabling high-throughput applications. Biophysical Reports , 1 (2). ISSN: 2667-0747 | doi: https://dx.doi.org/10.1016/j.bpr.2021.100028
Torre, E. , Bicer, M. , Cottrell, G. ORCID: 0000-0001-9098-7627 , Widera, D. ORCID: 0000-0003-1686-130X , Tamagnini, F. ORCID: 0000-0002-8741-5094 (2021) Time-dependent reduction of calcium oscillations in adipose-derived stem cells differentiating towards adipogenic and osteogenic lineage. Biomolecules , 11 (10). ISSN: 2218-273X | doi: https://dx.doi.org/10.3390/biom11101400
Pershina, O. , Ermakova, N. , Pakhomova, A. , Widera, D. ORCID: 0000-0003-1686-130X , Pan, E. , Zhukova, M. , Slonimskaya, E. , Morozov, S. , Kubatiev, A. , Dygai, A. , Skurikhin, E. (2021) Cancer stem cells and somatic stem cells as potential new drug targets, prognosis markers, and therapy efficacy predictors in breast cancer treatment. Biomedicines , 9 (9). ISSN: 2227-9059 | doi: https://dx.doi.org/10.3390/biomedicines9091223
Skurikhin, E. , Madonov, P. , Pershina, O. , Ermakova, N. , Pakhomova, A. , Widera, D. ORCID: 0000-0003-1686-130X , Pan, E. , Zhukova, M. , Sandrikina, L. , Artamonov, A. , Dygai, A. (2021) Micellar Hyaluronidase and Spiperone as a potential treatment for pulmonary fibrosis. International Journal of Molecular Sciences , 22 (11). ISSN: 1422-0067 | doi: https://dx.doi.org/10.3390/ijms22115599
Vaughan, D. , Mitchell, R. , Kretz, O. , Chambers, D. , Lalowski, M. , Amthor, H. , Ritvos, O. , Pasternack, A. , Matsakas, A. , Vaiyapuri, S. ORCID: 0000-0002-6006-6517 , Huber, T. , Denecke, B. , Mukherjee, A. , Widera, D. ORCID: 0000-0003-1686-130X , Patel, K. (2021) A muscle growth promoting treatment based on the attenuation of activin/myostatin signalling in young mice results in long-term testicular abnormalities. Disease Models & Mechanisms , 14 (2). ISSN: 1754-8411 | doi: https://dx.doi.org/10.1242/dmm.047555
Ridzuan, N. , Zakaria, N. , Widera, D. ORCID: 0000-0003-1686-130X , Sheard, J. , Morimoto, M. , Kiyokawa, H. , Azmel Mohd Isa, S. , Singh, G. , Then, K. , Ooi, G. , Yahaya, B. (2021) Human umbilical cord mesenchymal stem cell-derived extracellular vesicles ameliorate airway inflammation in a rat model of Chronic Obstructive Pulmonary Disease (COPD). Stem cell research and therapy , 12 (1). ISSN: 1757-6512 | doi: https://dx.doi.org/10.1186/s13287-020-02088-6
Bicer, M. , Cottrell, G. ORCID: 0000-0001-9098-7627 , Widera, D. ORCID: 0000-0003-1686-130X (2021) Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells. Stem cell research and therapy , 12 ISSN: 1757-6512 | doi: https://dx.doi.org/10.1186/s13287-020-02094-8
Dallas, M. ORCID: 0000-0002-5190-0522 and Widera, D. ORCID: 0000-0003-1686-130X (2021) TLR2 and TLR4-mediated inflammation in Alzheimer`s disease: self-defense or sabotage?. Neural Regeneration Research , 16 (8). pp. 1552-1553. ISSN: 1876-7958 | doi: https://dx.doi.org/10.4103/1673-5374.303016
Vallance, T. , Sheard, J. , Meng, Y. , Torre, E. , Patel, K. , Widera, D. ORCID: 0000-0003-1686-130X , Vaiyapuri, S. ORCID: 0000-0002-6006-6517 (2021) Development and characterisation of a novel, megakaryocyte NF-κB reporter cell line for investigating inflammatory responses. Journal of Thrombosis and Haemostasis , 19 (1). pp. 107-120. ISSN: 1538-7933 | doi: https://dx.doi.org/10.1111/jth.15118

Bicer, M. , Sheard, J. , Iandolo, D. , Boateng, S. , Cottrell, G. ORCID: 0000-0001-9098-7627 , Widera, D. ORCID: 0000-0003-1686-130X (2020) Electrical stimulation of adipose-derived stem cells in 3D nanofibrillar cellulose increases their osteogenic potential. Biomolecules , 10 (12). ISSN: 2218-273X | doi: https://dx.doi.org/10.3390/biom10121696
Skurikhin, E. , Nebolsin, V. , Widera, D. ORCID: 0000-0003-1686-130X , Ermakova, N. , Pershina, O. , Pakhomova, A. , Krupin, V. , Pan, E. , Zhukova, M. , Novikov, F. , Sandrikina, L. , Morozov, S. , Kubatiev, A. , Dygai, A. (2020) Antifibrotic and regenerative effects of Treamid in pulmonary fibrosis. International Journal of Molecular Sciences , 21 (21). ISSN: 1422-0067 | doi: https://dx.doi.org/10.3390/ijms21218380
Pakhomova, A. , Pershina, O. , Vladimir, N. , Natalia, E. , Vyacheslav, K. , Lubov, S. , Edgar, P. , Widera, D. ORCID: 0000-0003-1686-130X , Alexander, D. , Evgenii, S. (2020) Bisamide derivative of Dicarboxylic Acid contributes to restoration of testicular tissue function and influences spermatogonial stem cells in metabolic disorders. Frontiers in Cell and Developmental Biology , 8 ISSN: 2296-634X | doi: https://dx.doi.org/10.3389/fcell.2020.562358
Sanduk, F. , Meng, Y. , Widera, D. ORCID: 0000-0003-1686-130X , Kowalczyk, R. , Michael, N. , Kaur, A. , Yip, V. , Zulu, S. , Zavrou, I. , Hana, L. , Yaqoob, M. , Al-Obaidi, H. (2020) Enhanced anti-inflammatory potential of degradation resistant curcumin/ferulic acid eutectics embedded in triglyceride-based microemulsions. Journal of Drug Delivery Science and Technology , 60 ISSN: 1773-2247 | doi: https://dx.doi.org/10.1016/j.jddst.2020.102067
Ziegler, K. , Kunert, A. , Reinmuth-Selzle, K. , Leifke, A. , Widera, D. , Weller, M. , Schuppan, D. , Fröhlich-Nowoisky, J. , Lucas, K. , Pöschl, U. (2020) Chemical modification of pro-inflammatory proteins by peroxynitrite increases activation of TLR4 and NF-κB: implications for the health effects of air pollution and oxidative stress. Redox Biology , 37 ISSN: 2213-2317 | doi: https://dx.doi.org/10.1016/j.redox.2020.101581
Pakhomova, V. , Nebolsin, V. , Pershina, O. , Krupin, V. , Sandrikina, L. , Pan, E. , Ermakova, N. , Vaizova, O. , Widera, D. , Grimm, W. , Kravtsov, V. , Afanasiev, S. , Morozov, S. , Kubatiev, A. , Dygai, A. , Skurikhin, E. (2020) Antidiabetic effects of Bisamide Derivative of Dicarboxylic Acid in metabolic disorders. International Journal of Molecular Sciences , 21 (3). ISSN: 1422-0067 | doi: https://dx.doi.org/10.3390/ijms21030991
Vaughan, D. , Ritvos, O. , Mitchell, R. , Kretz, O. , Lalowski, M. , Amthor, H. , Chambers, D. , Matsakas, A. , Pasternack, A. , Collins-Hooper, H. , Ballesteros, R. , Huber, T. , Denecke, B. , Widera, D. , Mukherjee, A. , Patel, K. (2020) Inhibition of Activin/Myostatin signalling induces skeletal muscle hypertrophy but impairs mouse testicular development. European journal of translational myology , 30 (1). pp. 62-78. ISSN: 2037-7452 | doi: https://dx.doi.org/10.4081/ejtm.2019.8737
Iandolo, D. , Sheard, J. , Katarivas Levy, G. , Pitsalidis, C. , Tan, E. , Dennis, A. , Kim, J. , Markaki, A. , Widera, D. , Owens, R. (2020) Biomimetic and electroactive 3D scaffolds for human neural crest-derived stem cell expansion and osteogenic differentiation. MRS Communications , 10 (1). pp. 179-187. ISSN: 2159-6859 | doi: https://dx.doi.org/10.1557/mrc.2020.10
Karathanasis, C. , Medler, J. , Fricke, F. , Smith, S. , Malkusch, S. , Widera, D. , Fulda, S. , Wajant, H. , van Wijk, S. , Dikic, I. , Heilemann, M. (2020) Single-molecule imaging reveals the oligomeric state of functional TNFα-induced plasma membrane TNFR1 clusters in cells. Science Signaling , 13 (614). ISSN: 1937-9145 | doi: https://dx.doi.org/10.1126/scisignal.aax5647

Vallance, T. , Ravishankar, D. , Albadawi, D. , Layfield, H. , Sheard, J. , Vaiyapuri, R. , Dash, P. , Patel, K. , Widera, D. , Vaiyapuri, S. (2019) Effect of ultrapure lipopolysaccharides derived from diverse bacterial species on the modulation of platelet activation. Scientific Reports , 9 ISSN: 2045-2322 | doi: https://dx.doi.org/10.1038/s41598-019-54617-w
Pershina, O. , Pakhomova, A. , Widera, D. , Ermakova, N. , Epanchintsev, A. , Pan, E. , Krupin, V. , Vaizova, O. , Putrova, O. , Sandrikina, L. , Kurochkina, I. , Morozov, S. , Kubatiev, A. , Dygai, A. , Skurikhin, E. (2019) Gender differences in the pharmacological actions of pegylated glucagon-Like peptide-1 on endothelial progenitor cells and angiogenic precursor cells in a combination of metabolic disorders and lung emphysema. International Journal of Molecular Sciences , 20 (21). pp 0. ISSN: 1422-0067 | doi: https://dx.doi.org/10.3390/ijms20215414
Ridzuan, R. , Widera, D. , Yahaya, B. (2019) Secretome of mesenchymal stem cells and its impact on Chronic Obstructive Pulmonary Disease. In: Pham, P. , (eds.) Stem Cell Transplantation for Autoimmune Diseases and Inflammation. Stem Cells in Clinical Applications Springer pp. 139-157. ISBN: 978-3-030-23420-1 | doi: https://dx.doi.org/10.1007/978-3-030-23421-8_8
Sheard, J. , Bicer, M. , Meng, Y. , Frigo, A. , Martinez Aguilar, R. , Vallance, T. , Iandolo, D. , Widera, D. (2019) Optically transparent anionic nanofibrillar cellulose is cytocompatible with human adipose tissue-derived stem cells and allows simple imaging in 3D. Stem Cells International , 2019 ISSN: 1687-966X | doi: https://dx.doi.org/10.1155/2019/3106929
De Simone, A. , Naldi, M. , Tedesco, D. , Milelli, A. , Bartolini, M. , Davani, L. , Widera, D. , Dallas, M. , Andrisano, V. (2019) Investigating in vitro amyloid peptide 1-42 aggregation: impact of higher molecular weight stable adducts. ACS Omega , 4 (7). pp. 12308-12318. ISSN: 2470-1343 | doi: https://dx.doi.org/10.1021/acsomega.9b01531
Brown, P. , RELISH Consortium , Zhou, Y. , Widera, D. (2019) Large expert-curated database for benchmarking document similarity detection in biomedical literature search. Database , 2019 ISSN: 1758-0463 | doi: https://dx.doi.org/10.1093/database/baz085
Mitchell, R. , Mellows, B. , Sheard, J. , Antonioli, M. , Kretz, O. , Chambers, D. , Zeuner, M. , Tomkins, J. , Denecke, B. , Musante, L. , Joch, B. , Debacq-Chainiaux, F. , Holthofer, H. , Ray, S. , Huber, T. , Dengjel, J. , De Coppi, P. , Widera, D. , Patel, K. (2019) Secretome of adipose-derived mesenchymal stem cells promotes skeletal muscle regeneration through synergistic action of extracellular vesicle cargo and soluble proteins. Stem Cell Research and Therapy , 10 ISSN: 1757-6512 | doi: https://dx.doi.org/10.1186/s13287-019-1213-1
Gramignoli, R. , Sallustio, F. , Widera, D. , Raschzok, N. , eds. (2019) Tissue repair and regenerative mechanisms by stem/progenitor cells and their secretome. Frontiers in Medicine Frontiers
Widera, D. , Martinez-Aguilar, R. , Cottrell, G. (2019) Toll-like 4 and protease-activated receptor 2 in physiology and pathophysiology of the nervous system: more than just receptor cooperation?. Neural Regeneration Research , 14 (7). pp. 1196-1201. ISSN: 1876-7958 | doi: https://dx.doi.org/10.4103/1673-5374.251290
Haque, N. , Widera, D. , Abu Kasim, N. (2019) Stem cells from human extracted deciduous teeth expanded in foetal bovine and human sera express different paracrine factors after exposure to freshly prepared human serum. Advances in Experimental Medicine and Biology pp. 175-186. ISSN: 0065-2598 | doi: https://dx.doi.org/10.1007/5584_2018_299

Sheard, J. , Ramasamy, T. , Widera, D. (2018) From fibroblasts to medicinal signalling cells: a paradigm shift in translational stem cell research. Drug Target Review , 2018 (2). pp. 42-44. ISSN: 2059-1349
Zakaria, N. , Yusoff, N. , Zakaria, Z. , Widera, D. , Yahaya, B. (2018) Inhibition of NF-κB signalling reduces the stemness characteristics of lung cancer stem cells. Frontiers in Oncology , 8 ISSN: 2234-943X | doi: https://dx.doi.org/10.3389/fonc.2018.00166
Zeuner, M. , Didenko, N. , Humphries, D. , Stergiadis, S. ORCID: 0000-0002-7293-182X , Morash, T. , Patel, K. , Grimm, W. , Widera, D. ORCID: 0000-0003-1686-130X (2018) Isolation and characterisation of neural crest-derived stem cells from adult ovine palatal tissue. Frontiers in Cell and Developmental Biology , 6 ISSN: 2296-634X | doi: https://dx.doi.org/10.3389/fcell.2018.00039

Krüger, C. , Zeuner, M. , Cottrell, G. , Widera, D. , Heilemann, M. (2017) Quantitative single-molecule imaging of TLR4 reveals ligand-specific receptor dimerization. Science Signaling , 10 (503). ISSN: 1937-9145 | doi: https://dx.doi.org/10.1126/scisignal.aan1308
Vallance, T. , Zeuner, M. , Williams, H. , Widera, D. , Vaiyapuri, S. (2017) Toll-like receptor 4 signalling and its impact on platelet function, thrombosis, and haemostasis. Mediators of inflammation , 2017 ISSN: 0962-9351 | doi: https://dx.doi.org/10.1155/2017/9605894
Azoidis, I. , Metcalfe, J. , Reynolds, J. , Keeton, S. , Hakki, S. , Sheard, J. , Widera, D. (2017) Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels. MRS Communications , 7 (3). pp. 458-465. ISSN: 2159-6859 | doi: https://dx.doi.org/10.1557/mrc.2017.59
Mellows, B. , Mitchell, R. , Antonioli, M. , Kretz, O. , Chamber, D. , Zeuner, M. , Bernd, D. , Musante, L. , Durrgah, R. , Debacq-Chainiaux, F. , Holthofer, H. , Joch, B. , Ray, S. , Widera, D. , David, A. , Huber, T. , Dengjel, J. , De Coppi, P. , Patel, K. (2017) Protein and molecular characterization of a clinically compliant amniotic fluid stem cell derived extracellular vesicle fraction capable of accelerating muscle regeneration through the enhancement of angiogenesis. Stem Cells and Development , 26 (18). pp. 1316-1333. ISSN: 1547-3287 | doi: https://dx.doi.org/10.1089/scd.2017.0089
Zeuner, M. , Vallance, T. , Vaiyapuri, S. , Cottrell, G. , Widera, D. (2017) Development and characterisation of a novel NF-κB reporter cell line for investigation of neuroinflammation. Mediators of inflammation , 2017 ISSN: 0962-9351 | doi: https://dx.doi.org/10.1155/2017/6209865
Kardia, E. , Zakaria, N. , Halim, N. , Widera, D. , Yahaya, B. (2017) The use of mesenchymal stromal cells in treatment of lung disorders. Regenerative Medicine , 12 (2). pp. 2013-2016. ISSN: 1746-076X | doi: https://dx.doi.org/10.2217/rme-2016-0112

Zeuner, M. , Krueger, C. , Volk, K. , Bieback, K. , Cottrell, G. , Heilemann, M. , Widera, D. (2016) Biased signalling is an essential feature of TLR4 in glioma cells. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research , 1863 (12). pp. 3084-3095. ISSN: 0167-4889 | doi: https://dx.doi.org/10.1016/j.bbamcr.2016.09.016
Müller, J. , Greiner, J. , Zeuner, M. , Brotzmann, V. , Schäfermann, J. , Wieters, F. , Widera, D. , Sudhoff, H. , Kaltschmidt, B. , Kaltschmidt, C. (2016) 1,8-cineol potentiates IRF3-mediated antiviral response in human stem cells and an ex vivo model of rhinosinusitis. Clinical Science ISSN: 0143-5221 | doi: https://dx.doi.org/10.1042/CS20160218
Widera, D. , Klenke, C. , Nair, D. , Heidbreder, M. , Malkusch, S. , Sibarita, J. , Choquet, D. , Kaltschmidt, B. , Heilemann, M. , Kaltschmidt, C. (2016) Single-particle tracking uncovers dynamics of glutamate-induced retrograde transport of NF-κB p65 in living neurons. Neurophotonics , 3 (4). ISSN: 2329-4248 | doi: https://dx.doi.org/10.1117/1.NPh.3.4.041804
Zeuner, M. , Patel, K. , Denecke, B. , Giebel, B. , Widera, D. (2016) Paracrine effects of TLR4-polarised mesenchymal stromal cells are mediated by extracellular vesicles. Journal of Translational Medicine , 14 ISSN: 1479-5876 | doi: https://dx.doi.org/10.1186/s12967-016-0794-z

Grimm, W. , Giesenhagen, B. , Hakki, S. , Schau, I. , Sirak, S. , Sletov, A. , Varga, G. , Vukovic, M. , Widera, D. (2015) Translational research and therapeutic applications of neural crest-derived stem cells in regenerative periodontology. Current Oral Health Reports , 2 (4). pp. 266-274. ISSN: 2196-3002 | doi: https://dx.doi.org/10.1007/s40496-015-0067-6
Schürmann, M. , Frese, N. , Beyer, A. , Heimann, P. , Widera, D. , Mönkemöller, V. , Huser, T. , Barbara, K. , Christian, K. , Armin, G. (2015) Helium ion microscopy visualizes lipid nanodomains in mammalian cells. Small , 11 (43). pp. 5781-5789. ISSN: 1613-6810 | doi: https://dx.doi.org/10.1002/smll.201501540
Zeuner, M. , Bieback, K. , Widera, D. (2015) Controversial role of toll-like receptor 4 in adult stem cells. Stem cell reviews and reports , 11 (4). pp. 621-634. ISSN: 1558-6804 | doi: https://dx.doi.org/10.1007/s12015-015-9589-5
Müller, J. , Ossig, C. , Greiner, J. , Hauser, S. , Fauser, M. , Widera, D. , Kaltschmidt, C. , Storch, A. , Kaltschmidt, B. (2015) Intrastriatal transplantation of adult human neural crest-derived stem cells improves functional outcome in parkinsonian rats. Stem cells translational medicine , 4 (1). pp. 31-43. ISSN: 2157-6564 | doi: https://dx.doi.org/10.5966/sctm.2014-0078
Weber, M. , Apostolova, G. , Widera, D. , Mittelbronn, M. , Dechant, G. , Kaltschmidt, B. , Rohrer, H. (2015) Alternative generation of CNS neural stem cells and PNS derivatives from neural crest-derived peripheral stem cells. Stem Cells , 33 (2). pp. 574-588. ISSN: 1066-5099 | doi: https://dx.doi.org/10.1002/stem.1880

Schürmann, M. , Wolff, A. , Widera, D. , Hauser, S. , Heimann, P. , Hütten, A. , Kaltschmidt, C. , Kaltschmidt, B. (2014) Interaction of adult human neural crest-derived stem cells with a nanoporous titanium surface is sufficient to induce their osteogenic differentiation. Stem Cell Research , 13 (1). pp. 98-110. ISSN: 1876-7753 | doi: https://dx.doi.org/10.1016/j.scr.2014.04.017
Greiner, J. , Grunwald, L. , Müller, J. , Sudhoff, H. , Widera, D. , Kaltschmidt, C. , Kaltschmidt, B. (2014) Culture bag systems for clinical applications of adult human neural crest-derived stem cells. Stem cell research and therapy , 5 (2). ISSN: 1757-6512 | doi: https://dx.doi.org/10.1186/scrt422
Widera, D. , Müller, J. , Imielski, Y. , Heimann, P. , Kaltschmidt, C. , Kaltschmidt, B. (2014) Methods for the modulation and analysis of NF-κB-dependent adult neurogenesis. Journal of Visualized Experiments , 84 ISSN: 1940-087X | doi: https://dx.doi.org/10.3791/50870
Fricke, F. , Malkusch, S. , Wangorsch, G. , Greiner, J. , Kaltschmidt, B. , Kaltschmidt, C. , Widera, D. , Dandekar, T. , Heilemann, M. (2014) Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order oligomers. Histochemistry and cell biology , 142 (1). pp. 91-101. ISSN: 1432-119X | doi: https://dx.doi.org/10.1007/s00418-014-1195-0
Slotta, C. , Müller, J. , Tran, L. , Hauser, S. , Widera, D. , Kaltschmidt, B. , Kaltschmidt, C. (2014) An investigation of the specificity of research antibodies against NF-κB-subunit p65. Journal of histochemistry and cytochemistry , 62 (2). pp. 157-161. ISSN: 1551-5044 | doi: https://dx.doi.org/10.1369/0022155413515484

Nguyen, T. , Widera, D. , Greiner, J. , Müller, J. , Martin, I. , Slotta, C. , Hauser, S. , Kaltschmidt, C. , Kaltschmidt, B. (2013) Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells. Biological chemistry , 394 (12). pp. 1623-1636. ISSN: 1437-4315 | doi: https://dx.doi.org/10.1515/hsz-2013-0191
Greiner, J. , Müller, J. , Zeuner, M. , Hauser, S. , Seidel, T. , Klenke, C. , Grunwald, L. , Schomann, T. , Widera, D. , Sudhoff, H. , Kaltschmidt, B. , Kaltschmidt, C. (2013) 1,8-Cineol inhibits nuclear translocation of NF-κB p65 and NF-κB-dependent transcriptional activity. Biochimica Et Biophysica Acta-Molecular Cell Research , 1833 (12). pp. 2866-2878. ISSN: 0167-4889 | doi: https://dx.doi.org/10.1016/j.bbamcr.2013.07.001
Klenke, C. , Widera, D. , Engelen, T. , Müller, J. , Noll, T. , Niehaus, K. , Schmitz, M. , Kaltschmidt, B. , Kaltschmidt, C. (2013) Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE , 8 (6). ISSN: 1932-6203 | doi: https://dx.doi.org/10.1371/journal.pone.0065280
Sporbert, A. , Cseresnyes, Z. , Heidbreder, M. , Domaing, P. , Hauser, S. , Kaltschmidt, B. , Kaltschmidt, C. , Heilemann, M. , Widera, D. (2013) Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots. PLoS ONE , 8 (5). ISSN: 1932-6203 | doi: https://dx.doi.org/10.1371/journal.pone.0064023
Schomann, T. , Qunneis, F. , Widera, D. , Kaltschmidt, C. , Kaltschmidt, B. (2013) Improved method for ex ovo-cultivation of developing chicken embryos for human stem cell xenografts. Stem cells international , 2013 ISSN: 1687-966X | doi: https://dx.doi.org/10.1155/2013/960958
Widera, M. , Erkelenz, S. , Hillebrand, F. , Krikoni, A. , Widera, D. , Kaisers, W. , Deenen, R. , Gombert, M. , Dellen, R. , Pfeiffer, T. , Kaltschmidt, B. , Münk, C. , Bosch, V. , Köhrer, K. , Schaal, H. (2013) An intronic G run within HIV-1 intron 2 is critical for splicing regulation of vif mRNA. Journal of Virology , 87 (5). pp. 2707-2720. ISSN: 0022-538X | doi: https://dx.doi.org/10.1128/JVI.02755-12

Neugart, F. , Widera, D. , Kaltschmidt, B. , Kaltschmidt, C. , Heilemann, M. (2012) TNF receptor membrane dynamics studied with fluorescence microscopy and spectroscopy. Fluorescent Methods to Study Biological Membranes. , 13. Springer pp. 439-455. ISSN: 1617-1306 | doi: https://dx.doi.org/10.1007/4243_2012_60
Klenke, C. , Janowski, S. , Borck, D. , Widera, D. , Ebmeyer, J. , Kalinowski, J. , Leichtle, A. , Hofestädt, R. , Upile, T. , Kaltschmidt, C. , Kaltschmidt, B. , Sudhoff, H. (2012) Identification of novel cholesteatoma-related gene expression signatures using full-genome microarrays. PLoS ONE , 7 (12). ISSN: 1932-6203 | doi: https://dx.doi.org/10.1371/journal.pone.0052718
Widera, D. , Hauser, S. , Kaltschmidt, C. , Kaltschmidt, B. (2012) Origin and regenerative potential of vertebrate mechanoreceptor-associated stem cells. Anatomy research international , 2012 ISSN: 2090-2751 | doi: https://dx.doi.org/10.1155/2012/837626
Chakarov, N. , Greiner, J. , Hauser, S. , Schuetzmann, D. , Krüger, O. , Boerner, M. , Widera, D. (2012) Label-free enrichment of avian Leucocytozoon using flow cytometric sorting. Parasitology , 139 (12). pp. 1547-1552. ISSN: 0031-1820 | doi: https://dx.doi.org/10.1017/S0031182012000972
Heidbreder, M. , Zander, C. , Malkusch, S. , Widera, D. , Kaltschmidt, B. , Kaltschmidt, C. , Nair, D. , Choquet, D. , Sibarita, J. , Heilemann, M. (2012) TNF-α influences the lateral dynamics of TNF receptor I in living cells. Biochimica Et Biophysica Acta-Molecular Cell Research , 1823 (10). pp. 1984-1989. ISSN: 0167-4889 | doi: https://dx.doi.org/10.1016/j.bbamcr.2012.06.026
Martin, I. , Nguyen, T. , Krell, V. , Greiner, J. , Müller, J. , Hauser, S. , Heimann, P. , Widera, D. (2012) Generation of Schwann cell-derived multipotent neurospheres isolated from intact sciatic nerve. Stem cell reviews and reports , 8 (4). pp. 1178-1187. ISSN: 1558-6804 | doi: https://dx.doi.org/10.1007/s12015-012-9387-2
Kaltschmidt, B. , Kaltschmidt, C. , Widera, D. (2012) Adult craniofacial stem cells: sources and relation to the neural crest. Stem cell reviews and reports , 8 (3). pp. 658-671. ISSN: 1558-6804 | doi: https://dx.doi.org/10.1007/s12015-011-9340-9
Hauser, S. , Widera, D. , Qunneis, F. , Müller, J. , Zander, C. , Greiner, J. , Strauss, C. , Lüningschrör, P. , Heimann, P. , Schwarze, H. , Ebmeyer, J. , Sudhoff, H. , Araúzo-Bravo, M. , Greber, B. , Zaehres, H. , Schöler, H. , Kaltschmidt, C. , Kaltschmidt, B. (2012) Isolation of novel multipotent neural crest-derived stem cells from adult human inferior turbinate. Stem cells and development , 21 (5). pp. 742-756. ISSN: 1547-3287 | doi: https://dx.doi.org/10.1089/scd.2011.0419

Greiner, J. , Hauser, S. , Widera, D. , Müller, J. , Qunneis, F. , Zander, C. , Martin, I. , Mallah, J. , Schuetzmann, D. , Prante, C. , Schwarze, H. , Prohaska, W. , Beyer, A. , Rott, K. , Hütten, A. , Gölzhäuser, A. , Sudhoff, H. , Kaltschmidt, C. , Kaltschmidt, B. (2011) Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeutics. European cells & materials , 22 pp. 403-419. ISSN: 1473-2262
Widera, D. , Heimann, P. , Zander, C. , Imielski, Y. , Heidbreder, M. , Heilemann, M. , Kaltschmidt, C. , Kaltschmidt, B. (2011) Schwann cells can be reprogrammed to multipotency by culture. Stem cells and development , 20 (12). pp. 2053-2064. ISSN: 1547-3287 | doi: https://dx.doi.org/10.1089/scd.2010.0525

Heidbreder, M. , Endesfelder, U. , van de Linde, S. , Hennig, S. , Widera, D. , Kaltschmidt, B. , Kaltschmidt, C. , Heilemann, M. (2010) Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. Biochimica Et Biophysica Acta-Molecular Cell Research , 1803 (10). pp. 1224-1229. ISSN: 0167-4889 | doi: https://dx.doi.org/10.1016/j.bbamcr.2010.06.004
Arnold, W. , Becher, S. , Dannan, A. , Widera, D. , Dittmar, T. , Jacob, M. , Mannherz, H. , Dittmar, T. , Kaltschmidt, B. , Kaltschmidt, C. , Grimm, W. (2010) Morphological characterization of periodontium-derived human stem cells. Annals of Anatomy - Anatomischer Anzeiger , 192 (4). pp. 215-219. ISSN: 1618-0402 | doi: https://dx.doi.org/10.1016/j.aanat.2010.05.004
Kaus, A. , Widera, D. , Kassmer, S. , Peter, J. , Zaenker, K. , Kaltschmidt, C. , Kaltschmidt, B. (2010) Neural stem cells adopt tumorigenic properties by constitutively activated NF-kappaB and subsequent VEGF up-regulation. Stem cells and development , 19 (7). pp. 999-1015. ISSN: 1547-3287 | doi: https://dx.doi.org/10.1089/scd.2009.0416

Widera, D. , Kaltschmidt, C. , Kaltschmidt, B. (2009) NF-κB – potential role in adult neural stem cells. Encyclopedia of Neuroscience. Springer pp. 2862-2867. | doi: https://dx.doi.org/10.1007/978-3-540-29678-2_3971
Widera, D. , Zander, C. , Heidbreder, M. , Kasperek, Y. , Noll, T. , Seitz, O. , Saldamli, B. , Sudhoff, H. , Sader, R. , Kaltschmidt, C. , Kaltschmidt, B. (2009) Adult palatum as a novel source of neural crest-related stem cells. Stem Cells , 27 (8). pp. 1899-1910. ISSN: 1066-5099 | doi: https://dx.doi.org/10.1002/stem.104

Widera, D. , Kaus, A. , Kaltschmidt, C. , Kaltschmidt, B. (2008) Neural stem cells, inflammation and NF-kappaB: basic principle of maintenance and repair or origin of brain tumours?. Journal of Cellular and Molecular Medicine , 12 (2). pp. 459-470. ISSN: 1582-4934 | doi: https://dx.doi.org/10.1111/j.1582-4934.2007.00208.x

Moebius, J. , Widera, D. , Schmitz, J. , Kaltschmidt, C. , Piechaczek, C. (2007) Impact of polysialylated CD56 on natural killer cell cytotoxicity. BMC immunology , 8 (1). ISSN: 1471-2172 | doi: https://dx.doi.org/10.1186/1471-2172-8-13
Mikenberg, I. , Widera, D. , Kaus, A. , Kaltschmidt, B. , Kaltschmidt, C. (2007) Transcription factor NF-kappaB is transported to the nucleus via cytoplasmic dynein/dynactin motor complex in hippocampal neurons. PLoS ONE , 2 (7). ISSN: 1932-6203 | doi: https://dx.doi.org/10.1371/journal.pone.0000589
Widera, D. , Grimm, W. , Moebius, J. , Mikenberg, I. , Piechaczek, C. , Gassmann, G. , Wolff, N. , Thévenod, F. , Kaltschmidt, C. , Kaltschmidt, B. (2007) Highly efficient neural differentiation of human somatic stem cells, isolated by minimally invasive periodontal surgery. Stem cells and development , 16 (3). pp. 447-60. ISSN: 1547-3287 | doi: https://dx.doi.org/10.1089/scd.2006.0068

Kaltschmidt, B. , Mikenberg, I. , Widera, D. , Kaltschmidt, C. (2006) The role of NF-κB in brain function. Transcription Factors in the Nervous System. Wiley-VCH pp. 327-352. | doi: https://dx.doi.org/10.1002/3527608036.ch16
Piotrowska, M. , Widera, D. , Kaltschmidt, B. , an der Heiden, U. , Kaltschmidt, C. (2006) Mathematical model for NF-kappaB-driven proliferation of adult neural stem cells. Cell proliferation , 39 (6). pp. 441-55. ISSN: 0960-7722 | doi: https://dx.doi.org/10.1111/j.1365-2184.2006.00403.x
Widera, D. , Mikenberg, I. , Elvers, M. , Kaltschmidt, C. , Kaltschmidt, B. (2006) Tumor necrosis factor alpha triggers proliferation of adult neural stem cells via IKK/NF-kappaB signaling. BMC Neuroscience , 7 (1). ISSN: 1471-2202 | doi: https://dx.doi.org/10.1186/1471-2202-7-64
Lindecke, A. , Korte, M. , Zagrebelsky, M. , Horejschi, V. , Elvers, M. , Widera, D. , Prüllage, M. , Pfeiffer, J. , Kaltschmidt, B. , Kaltschmidt, C. (2006) Long-term depression activates transcription of immediate early transcription factor genes: involvement of serum response factor/Elk-1. The European Journal of Neuroscience , 24 (2). pp. 555-63. ISSN: 0953-816X | doi: https://dx.doi.org/10.1111/j.1460-9568.2006.04909.x
Widera, D. , Mikenberg, I. , Kaus, A. , Kaltschmidt, C. , Kaltschmidt, B. (2006) Nuclear Factor-kappaB controls the reaggregation of 3D neurosphere cultures in vitro. European cells & materials , 11 pp. 76-85. ISSN: 1473-2262
Mikenberg, I. , Widera, D. , Kaus, A. , Kaltschmidt, B. , Kaltschmidt, C. (2006) TNF-alpha mediated transport of NF-kappaB to the nucleus is independent of the cytoskeleton-based transport system in non-neuronal cells. European Journal of Cell Biology , 85 (6). pp. 529-36. ISSN: 0171-9335 | doi: https://dx.doi.org/10.1016/j.ejcb.2006.02.007
Widera, D. , Mikenberg, I. , Kaltschmidt, B. , Kaltschmidt, C. (2006) Potential role of NF-kappaB in adult neural stem cells: the underrated steersman?. International journal of developmental neuroscience , 24 (2-3). pp. 91-102. ISSN: 0736-5748 | doi: https://dx.doi.org/10.1016/j.ijdevneu.2005.11.017

Kaltschmidt, B. , Widera, D. , Kaltschmidt, C. (2005) Signaling via NF-kappaB in the nervous system. Biochimica Et Biophysica Acta-Molecular Cell Research , 1745 (3). pp. 287-99. ISSN: 0167-4889 | doi: https://dx.doi.org/10.1016/j.bbamcr.2005.05.009

Widera, D. , Holtkamp, W. , Entschladen, F. , Niggemann, B. , Zänker, K. , Kaltschmidt, B. , Kaltschmidt, C. (2004) MCP-1 induces migration of adult neural stem cells. European Journal of Cell Biology , 83 (8). pp. 381-7. ISSN: 0171-9335 | doi: https://dx.doi.org/10.1078/0171-9335-00403

Darius Widera

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