Dr Deepa Paliwal - Department of Biomedical Sciences
Areas of interest
- Passionate, motivated scientist, working as Postdoctoral Research Assistant, with experience and interest in Microbiology, Molecular Biology and Immunology.
- Research areas include Molecular Microbiology, Animal biotechnology, Genomics, and proteomics.
- Molecular Biology & Biochemistry: Gateway cloning, Real Time-PCR, DNA/RNA-Isolation, Purification and Estimation of protein, Western Blot, Protein purification and other biochemical assays.
- Microbiological: Fluorescence Microscopy, Confocal Microscopy, Maintenance of Pure Culture, Identification and Characterization of Microbes, various staining techniques.
- Plant Biotechnology: Plant Tissue Culture, Agrobacterium Mediated transformation, Transient Gene Expression.
- Animal Biotechnology: Maintenance of Mammalian & Insect Cell Culture, Transfection, Cell based Assays & baculovirus expression.
- Bioprocess Engineering: Batch Submerged Fermentation, Solid State Fermentation.
- Immunology: ELISA, Immunodiffusion Techniques, Western Blotting.
- Genetic Engineering: Restriction Digestion, Ligation, Competence cell preparation and Transformation, Southern & Northern Hybridization, Colony Hybridization.
- Bioinformatics: Next generation RNA & DNA sequencing at Galaxy, Tuxedo, Qiime and CLC work bench Platform Database Search, BLAST, ExPASy, PANTHER, Phylogenetic Analysis.
My Ph.D. at University of Reading explored underlying molecular mechanisms on how bacteria pathogens can kill insects with the aim of utilizing them as effective biocontrol agents. I have employed dual host-pathogen transcriptomic profiling which allows me to analyse large-scale transcriptomic data (RNA-Seq) through the use of Galaxy and another tuxedo platform. Moreover, various statistical tools on Linux & R program help me understand complex data and allowed me to interpret and present RNA & DNA sequencing data for publication work.
- Research assistant, Rothamsted Research, Harpenden,U.K. (June-12 to Sept-12)
- Academic visitor, University of Reading, Reading, UK.(Jan-12 to May-12)
- Research assistant, John Innes Centre, Norwich, U.K. (Mar-10 to Nov-11)
Academic credentials include double post-graduation in Biotechnology and Ph.D. in Biological Sciences.
Awards and honours
- Awarded University of Reading international studentship (Sept 2012 - 2017) focused on development of new biocontrol agents against aphids.
- Awarded In Qualified and was offered several National level Junior Research Fellowship (JRF), India 2007 - 2009
- Qualified National level Graduate Aptitude Test for Engineering (GATE) - 2007 with 13th rank & 99.88 percentile.
- Gold medal for securing first position in university during M.Tech Biotechnology.
- Scholarship from Department of Biotechnology, (Ministry of Science and Technology, Govt. of India) for both the Masters program (M.Sc & M.Tech)
- Received scholarship for holding first position in the entire college for my Bachelor of Science (B.Sc.) grade
Foster, S. P., Paliwal, D., Martin, J., & Williamson, M. S. (2012). Evolution of knock-down resistance to pyrethroids in grain aphids (Sitobion avenae) in the UK. Aspects of Applied Biology, (No.117), 95-96.
- Paliwal, D. ORCID: 0000-0002-6891-0778 , Thom, M. , Hussein, A. , Ravishankar, D. , Wilkes, A. , Charleston, B. , Jones, I. ORCID: 0000-0002-7738-2516 (2022) Towards reverse vaccinology for Bovine TB: high throughput expression of full length recombinant Mycobacterium bovis proteins. Frontiers in Molecular Biosciences , 9 ISSN: 2296-889X | doi: https://dx.doi.org/10.3389/fmolb.2022.889667
- Paliwal, D. ORCID: 0000-0002-6891-0778 , Hamilton, A. , Barrett, G. ORCID: 0000-0003-1509-0179 , Alberti, F. , Van Emden, H. , Monteil, C. , Machuline, T. , Nauen, R. , Wagstaff, C. ORCID: 0000-0001-9400-8641 , Bass, C. , Jackson, R. (2022) Identification of novel aphid-killing bacteria to protect plants. Microbial Biotechnology , 15 (4). pp. 1203-1220. ISSN: 1751-7915 | doi: https://dx.doi.org/10.1111/1751-7915.13902