Dr Laurence Bindschedler
Research
Within the Proteomics Unit of the :
- Advising and guiding users and staff : Use of mass spectrometry for proteomic purposes, protein sample fractionation, Data analysis.
- Maintenance of a nanoLC system and mass spectrometer instrumentation.
- Setting up workflows to analyse proteomic data.
- Coordinating the RMPMI (Reading Molecular Plant Microbe Interaction) interest group meeting.
Plant Proteomics:
I have established mass spectrometric techniques for the quantitative proteomics analysis in plants using N-15 metabolic labelling. I have an ongoing project/collaboration to establish fractionation methods to avoid the problem of high dynamic range of protein concentrations inherent to plant protein samples. Located in the proteom
Plant Pathology and Proteomics
In general terms I am interested in understanding interactions between plants and pathogens at the molecular and protein levels.
I am currently investigating the proteome of Blumeria graminis, an obligate fungal pathogen from barley. These data will be useful to annotate the genome of this newly sequenced fungus, and to understand the biotrophy of the pathogen.
I am also interested in studying proteome changes as the conscequence of active oxygen species production in plants submitted to biotic and abiotic stresses, in particular Pseudomonas syringae
Plant cell wall bionsynthesis
Secondary cell walls are the main source of cellulose and bio-fuels. To study secondary cell wall biosynthesis, I am investigated the possibility to set up a new in vitro cell culture system able to produce secondary cell walls.
Other publications
Palmblad M, Mills DJ, Bindschedler LV. 2008. Heat Shock Response in A. thaliana Explored by Multiplexed Quantitative Proteomics Using Differential Metabolic Labeling. Journal of Proteome Research 7:780-5
Bindschedler LV, Tuerck J, Maunders M, Ruel K,Petit-Conil M, Danoun S, Boudet AM, Joseleau JP, Bolwell GP. 2007. Modification of hemicellulose content by antisense down-regulation of UDP-glucuronate decarboxylase in tobacco and its conscequence for cellulose extactability. Phytochemistry 68: 2635-48.
Bindschedler LV, Dewdney J, Blee KA, Stone JM, Asai T, Plotnikov J, Denoux C, Hayes T, Gerrish C, Davies DR, Ausubel FM, Bolwell GP. 2006. Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance. Plant J. 47: 851-863.
Bindschedler LV, Whitelegge J, Millar D., Trethowan J, Bolwell GP. 2006. A two component chitin-binding protein from French bean – association of proline-rich protein with a cysteine-rich polypeptide.FEBS Lett. 580: 1541-1546.
Davies DR, Bindschedler LV,Strickland TS , Bolwell GP.2006. Production of reactive oxygen species in Arabidopsis thaliana cell suspension cultures in response to an elicitor from Fusarium oxysporum: implications for basal resistance. J Exp. Bot. 57: 1817-1827.
Bindschedler LV, Wheatley E,Gay E, Cole J, Cottage A,Bolwell GP. 2005. Characterisation and regulation of the pathway from UDP-glucose to UDP-xylose in differentiating tobacco tissue. Plant Molecular Biology.57 :285-301.
Bindschedler L, Sanchez P, Dunn S., Mikan J, Thangavelu, M, Clarkson JM, Cooper RM. 2003.Deletion of the SNP1 trypsin protease from Stagonospora nodorum reveals another major protease expressed during infection. Fungal Genetics and Biology, 38: 43-53.
Bolwell GP, Bindschedler LV, Blee KA, Butt, VS, Davies DR, Gardner SL, Gerrish C, Minibayeva F. 2002. The apoplastic oxidative burst in response to biotic stress in plants: a three-component system. Journal of Experimental Botany (Antioxidants and reactive oxygen species in plants special issue) 53, 1367-1376.
Bindschedler LV, Minibayeva F, Gardner SL, Gerrish C, Davies DR, Bolwell GP. 2001. Early signalling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca2+.New Phytologist 151:185-194.
14.Carlile A, Bindschedler L, Bailey AM, Bowyer P, Clarkson JM, Cooper RM. 2000.Characterisation of SNP1, a cell wall degrading trypsin, produced during infection by Stagonospora nodorum. Molecular Plant-Microbe Interaction 13: 538-550.
Vallélian-Bindschedler L, Mösinger E, Métraux JP, Schweizer P. 1998. Structure, expression and localization of a germin-like protein in barley (Hordeum vulgare L.) that is insolubilized in stressed leaves. Plant Molecular Biology 37: 297-308.
Vallélian-Bindschedler L, Métraux JP Schweizer P. 1998. Salicylic acid accumulation in barley is pathogen specific but not required for defence-gene activation. Molecular Plant-Microbe Interactions 11:702-705
Vallélian-Bindschedler L, Schweizer P, Mösinger E, Métraux JP. 1998. Heat-induced resistance in barley to powdery mildew (Blumeria graminis. f. sp. hordei) is associated with a burst of active oxygen species. Physiological and Molecular Plant Pathology 52: 185-199.
Schweizer P, Vallélian-Bindschedler L, Mösinger E. 1995. Heat induced resistance in barley to the powdery mildew fungus Erysiphe graminis f.sp. hordei.Physiological and Molecular Plant Pathology 47: 51-66.
Publications
- Pennington, H. , Gheorghe, D. , Damerum, A. , Pliego, C. , Spanu, P. , Cramer, R. , Bindschedler, L. (2016) Interactions between the powdery mildew effector BEC1054 and barley proteins identify candidate host targets. Journal of Proteome Research , 15 (3). pp. 826-839. ISSN: 1535-3907 | doi: https://dx.doi.org/10.1021/acs.jproteome.5b00732
- Agrawal, G. , Sarkar, A. , Righetti, P. , Pedreschi, R. , Carpentier, S. , Wang, T. , Barkla, B. , Kohli, A. , Ndimba, B. , Bykova, N. , Rampitsch, C. , Zolla, L. , Rafudeen, M. , Cramer, R. , Bindschedler, L. , Tsakirpaloglou, N. , Job, D. , Kikuchi, S. , Rakwal, R. (2013) A decade of plant proteomics and mass spectrometry: translation of technical advancements to food security and safety issues. Mass Spectrometry Reviews , 32 (5). pp. 335-365. ISSN: 1098-2787 | doi: https://dx.doi.org/10.1002/mas.21365
- Bindschedler, L. and Cramer, R. (2011) Fully automated software solution for protein quantitation by global metabolic labeling with stable isotopes. Rapid Communications in Mass Spectrometry , 25 (11). pp. 1461-1471. ISSN: 1097-0231 | doi: https://dx.doi.org/10.1002/rcm.4872
- Bindschedler, L. and Cramer, R. (2011) Quantitative plant proteomics. PROTEOMICS , 11 (4). pp. 756-775. ISSN: 1615-9861 | doi: https://dx.doi.org/10.1002/pmic.201000426
- Bindschedler, L. , McGuffin, L. , Burgis, T. , Spanu, P. , Cramer, R. (2011) Proteogenomics and in silico structural and functional annotation of the barley powdery mildew Blumeria graminis f. sp. hordei. Methods , 54 (4). pp. 432-441. ISSN: 1046-2023 | doi: https://dx.doi.org/10.1016/j.ymeth.2011.03.006
- Bindschedler, L. , Smith, C. , Cramer, R. (2011) Quantitative plant proteomics using hydroponic isotope labeling of entire plants (HILEP). In: Ivanov, A. and Lazarev, A. , (eds.) Sample preparation in biological mass spectrometry. , XVI. Springer , New York. ISBN: 9789400707580
- Spanu, P. , Abbott, J. , Amselem, J. , Burgis, T. , Soanes, D. , Stuber, K. , Loren van Themaat, E. , Brown, J. , Butcher, S. , Gurr, S. , Lebrun, M. , Ridout, C. , Schulze-Lefert, P. , Talbot, N. , Ahmadinejad, N. , Ametz, C. , Barton, G. , Benjdia, M. , Bidzinski, P. , Bindschedler, L. , Both, M. , Brewer, M. , Cadle-Davidson, L. , Cadle-Davidson, M. , Collemare, J. , Cramer, R. , Frenkel, O. , Godfrey, D. , Harriman, J. , Hoede, C. , King, B. , Klages, S. , Kleemann, J. , Knoll, D. , Koti, P. , Kreplak, J. , Lopez-Ruiz, F. , Lu, X. , Maekawa, T. , Mahanil, S. , Micali, C. , Milgroom, M. , Montana, G. , Noir, S. , O'Connell, R. , Oberhaensli, S. , Parlange, F. , Pedersen, C. , Quesneville, H. , Reinhardt, R. , Rott, M. , Sacristan, S. , Schmidt, S. , Schon, M. , Skamnioti, P. , Sommer, H. , Stephens, A. , Takahara, H. , Thordal-Christensen, H. , Vigouroux, M. , Wessling, R. , Wicker, T. , Panstruga, R. (2010) Genome expansion and gene loss in powdery mildew fungi reveal tradeoffs in extreme parasitism. Science , 330 (6010). pp. 1543-1546. ISSN: 1095-9203 | doi: https://dx.doi.org/10.1126/science.1194573
- Bindschedler, L. , Towers, M. , Cramer, R. (2009) Hydroponic isotope labeling of entire plants (HILEP) for quantitative plant proteomics.
- Bindschedler, L. , Burgis, T. , Mills, D. , Ho, J. , Spanu, P. , Cramer, R. (2009) In planta proteomics and proteogenomics of the biotrophic barley fungal pathogen blumeria f.sp. hordei.
- Boschetti, . , Bindschedler, . , Tang, . , Fasoli, . , Righetti, . (2009) Combinatorial peptide ligand libraries and plant proteomics: a winning strategy at a price. Journal of Chromatography A , 1216 (8). pp. 1215-1222. ISSN: 0021-9673 | doi: https://dx.doi.org/10.1016/j.chroma.2008.11.098
- Bindschedler, L. , Burgis, T. , Mills, D. , Ho, J. , Cramer, R. , Spanu, P. (2009) In planta proteomics and proteogenomics of the biotrophic barley fungal pathogen blumeria graminis f. sp hordei. Molecular & Cellular Proteomics , 8 (10). pp. 2368-2381. ISSN: 1535-9476 | doi: https://dx.doi.org/10.1074/mcp.M900188-MCP200
- Bindschedler, . , Palmblad, . , Cramer, . (2008) Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study. Phytochemistry , 69 (10). pp. 1962-1972. ISSN: 0031-9422 | doi: https://dx.doi.org/10.1016/j.phytochem.2008.04.007
- Bindschedler, . and Cramer, . (2008) Equalisation of protein amounts for overcoming the high dynamic protein expression range in the analysis of plant proteomes. pp. MP 573.
- Palmblad, . , Mills, D. , Bindschedler, . , Cramer, . (2007) Chromatographic alignment of LC-MS and LC-MS/MS datasets by genetic algorithm feature extraction. Journal of the American Society for Mass Spectrometry , 18 (10). pp. 1835-1843. ISSN: 1044-0305 | doi: https://dx.doi.org/10.1016/j.jasms.2007.07.018
- Palmblad, . , Bindschedler, . , Cramer, . (2007) Quantitative proteomics using uniform N-15-labeling, MASCOT, and the trans-proteomic pipeline. Proteomics , 7 (19). pp. 3462-3469. ISSN: 1615-9853 | doi: https://dx.doi.org/10.1002/pmic.200700180
- Bindschedler, L. , Palmblad, M. , Cramer, R. (2006) First-dimension separation with the MicroRotoforTM cell prior to SDS-PAGE and LC-MS/MS analysis. Bio-Rad Bulletin , 5451 pp. 1-4.
- Palmblad, M. , Bindschedler, L. , Gibson, T. , Cramer, R. (2006) Comparison of bottom-up protein identification methods.
- Palmblad, . , Bindschedler, . , Gibson, T. , Cramer, . (2006) Automatic internal calibration in liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry of protein digests. Rapid Communications in Mass Spectrometry , 20 (20). pp. 3076-3080. ISSN: 0951-4198 | doi: https://dx.doi.org/10.1002/rcm.2707