Thermal Analysis

ThermalAnalysis

The thermal analysis laboratory, housed within the CAF lab, contains a suite of high-performance instrumentation that can investigate thermal events within a wide range of materials, from archaeological, through to food and pharmaceutical samples. The facility houses the following equipment.

Mettler Toledo Hot Stage Microscope

The Mettler-Toledo hot/cold stage enables video and still imaging of thermal events of a variety of samples. With liquid nitrogen cooling it can look at a wide range of temperatures and sensitively control heating and cooling rates. In addition to thermal events, the microscope can also capture images, allows sizing of micro-objects and can record videos to observe kinetics of a variety of physical processes; for example, to investigate swelling kinetics of polymeric samples.

TA-Nano DSC (Solution Differential Scanning Microcalorimetry)

This DSC can be used to investigate dilute biological and polymeric aqueous solutions. It operates in a temperature range of -10 to 130 ºC with scan rates of 0.0001 up to 2 ºC/min. It is suitable for macromolecule concentrations in the range 0.03 to 1 mg/ml and requires 800 µl of sample per run (i.e. nanomoles). It determines stability as a function of temperature and allows determination of transition temperatures and enthalpies for 2-state unfolding events. It is ideal for investigating the unfolding characteristics of macromolecules and is also suitable for probing binding events.

TA-Q2000 DSC

A research grade instrument ideal for analysis of pharmaceuticals and polymeric materials. Fast and easy to use with autosampler. Modulation capability ideal for polymeric samples. Capable of scanning at rates up to 200 ºC/min to enable kinetic mediated transitions to be investigated.

TA-Q600SDT TGA (Thermal Gravimetric Analysis with DSC)

This instrument heats from room temperature to high temperatures and is ideal for analysis of inorganic samples. It provides TGA and DSC thermograms simultaneously.

Mictocal ITC200 (Isothermal Titration Calorimetry)

These instruments enable macromolecule-ligand binding interactions to be investigated to provide binding constants, stoichiometry of binding and thermodynamic parameters. A variety of systems can be investigated including protein-ligand binding, DNA interactions, deaggregation processes and molecular binding to lipid vesicles. Low volume sample cells enable small amounts of samples to be used.

For further details contact

Please contact

Hisham Al-Obaidi

caf.thermal@reading.ac.uk

 

 Thermal Analysis Section Head

Page navigation

 

Search Form

A-Z lists