Bruker DPX 400 MHz

NMR 400The DPX400 is a two-channel NMR instrument running ICON NMR 2.1 under TOPSPIN 1.3. It incorporates a 9.39 T magnet without active shielding and is equipped with a 5mm z-gradient direct observation (1H/13C/19F)- BBFO probe and a 60-position BACS sample changer

The DPX400 is an Open Access NMR Instrument, operating 24 hours a day, 7 days a week, with the exception of Mondays between 9AM-12 noon, which is reserved for instrument servicing and maintenance by the NMR Staff. The DPX400 is available only to research workers from the University of Reading. All users must have received Training in Use of the Open Access NMR Instruments (provided by the NMR Staff). When using the Open Access NMR Instruments you are expected to perform NMR Sample Preparation yourself.

By default, six ICONNMR Experiments: 1H (1D-), 13C (1D-), DEPT-135 (1D-), Edited-HSQC (2D-), HMBC (2D-) and COSY (2D-) are made available to all new users of the DPX400. However, the DPX400 can also run direct-detection experiments from many other nuclei, such as 31P, 19F and 15N. If you require these or other experiments, then please consult with the NMR Staff who will add them to your user account.

Of the two Open Access NMR instruments, the DPX400 is superior for performing heteronuclear direct detection, as the probe for this instrument has optimized sensitivity for heteronuclei. Therefore, if you have a limited amount of sample (see Sample Requirements for Experiments in NMR for the minimum amount of sample which is required in order for each of the above experiments to succeed) you will get much better results from running your 1D-NMR 13C, DEPTQ and DEPT-135 experiments on the DPX400, in preference to the Nano400. Conversely, 2D-NMR experiments involving direct observation of proton, such as edited-HSQC, HMBC, COSY and NOESY, will give better results on the DPX400, if only a small amount of sample is available. Very small amounts of sample, which cannot be run in a reasonable time on either of the Open Access NMR instruments, can be submitted to the Internal NMR Service (either the Bruker Avance III 500 MHz or the AV700 NMR instruments) for acquisition by the NMR Staff.

Health and Safety in the NMR Lab [PDF 302kb]

Training is provided to ensure you can use the instrument safe and effective:

  • NMR Training [PDF 259kb]

  • NMR guidance and support
  • More information

    For more information about the instrument and its availability contact:

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