Improving baculovirus expression. Funded by the
BBSRC this work focused on development of high throughput
expression systems. The aim was to reduce the bottleneck in protein
production currently preventing high throughout structure
determination. Our approach, an expression suite
of vectors allows expression in a variety of contexts and partly understands
what underpins the observed high and low expression levels of
certain proteins. Graduate students still involved are
Stuart Pengelley and Fredelina Yong.
SPINE - Structural Proteomics in Europe. A
European consortium lead by Oxford where we provided input
on high throughput baculovirus expression technology. SPINE has a large website
where you can find out about the technologies used, the targets
identified and the progress to date on crystal structures.
Virus display and the isolation of novel molecules.
Funded by the BBSRC as
part of its ExGen programme this project used
advanced baculovirus engineering for the display of cDNA libraries
on the surface of virus particles. We invented baculovirus display
in 1995 (Boublik etal) at the same time as
University of Natural
Resources and Applied Life Sciences, Vienna.
at the University of Jyväskylä in Finland has also worked on display.
A complex library and
develop panning procedures for the isolation of novel ligand
binding proteins were developed in a collaboration with the University
of Warwick Dept of