Baculovirus technology. Most of the tips and
hints we can offer on the use of baculoviruses for the expression of
recombinant proteins are on the Vector pages. In addition there are
lots of pages on the web with details of the various protocols used.
An overview of the system by the inventor himself is available on
the Protein Sciences
website although he has recently moved on to be VP of Vaccine
Development at Novavax. Some recent pages from Laurence Pearl's group at
the Institute of Cancer Research (aka
Domainex) are good (here) and overviews of the systems and their
history are available from various commercial suppliers (below).
Beware the hype however, Seakem agarose is as good as any "special"
insect cell grade for plaque assays and most transfection agents work well with
insect cells so you don't need a special reagent. The real secret is
thoughtful construct design followed by good virology and good insect
cell tissue culture, all essentially free.
Some commercial suppliers with relevant information
pages or pdfs are:
For a general description of baculoviruses we
recommend the excellent
provided by Alan Cann at the University of Leicester.
For general information on viruses use the
All the Virology on the Web
site as a leaping off point for searching for what you want.
The graphics and all our manuscript artwork use the amazing (and very reasonably priced) vector drawing programme
Don't want to pay for any commercial software? We
advise the excellent and free OpenOffice suite
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biology and evolution? Go
find like minded souls.