Staff Profile:Professor Phil Knight

Name:

Professor Phil Knight

Job Title:

Academic, Director of Research, Hopkins Building

Responsibilities:

Areas of Interest:

In broad terms my research group aims to increase understanding of the mechanisms that regulate ovarian follicular development, ovulation and early embryo development in mammals and birds.

Over the years we have conducted research involving each level of the hypothalamic-pituitary-ovarian axis but more recently our attention has focused on the roles of intraovarian peptides as local and systemic regulators of follicle development. We have a particular interest in the physiological roles of inhibins, activins and follistatins in this regard and have developed a range of highly sensitive and specific analytical methods to detect and quantify these proteins in biological samples. Our research makes extensive use of in vitro models involving isolated animal cells and tissues in primary culture but an in vivo approach (e.g. serum hormone profiling, ovarian ultrasound scanning, ovarian cycle manipulation) is also used in some animal studies and in most of our collaborative studies on human subjects.

As well as being of fundamental scientific interest, it is anticipated that our research will underpin the development of improved methods for manipulating reproduction and for diagnosing and treating infertility in animals and man.

Research groups / Centres:

Development and endocrinology research group

Publications:

Y

Jump to: 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003

Number of items: 34.

2013

• Satchell, L., Glister, C., Bleach, E. C., Glencross, R., Bicknell, A., Dai, Y., Anand-Ivell, R., Ivell, R. and Knight, P. (2013) Ovarian expression of insulin-like peptide 3 (INSL3) and its receptor (RXFP2) during development of bovine antral follicles and corpora lutea and measurement of circulating INSL3 levels during synchronized estrous cycles. Endocrinology. ISSN 1945-7170 (In Press)
• Glister, C., Satchell, L., Bathgate, R. J., Wade, J. D., Dai, Y., Ivell, R., Anand-Ivell, R., Rodgers, R. J. and Knight, P. (2013) A functional link between bone morphogenetic proteins and insulin-like peptide 3 signaling in modulating ovarian androgen production. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424 (In Press)

2012

• Glister, C., Satchell, L., Michael, A. E., Bicknell, A. B. and Knight, P. G. (2012) The anti-epileptic drug Valproic Acid (VPA) inhibits steroidogenesis in bovine theca and granulosa cells in vitro. PLoS ONE, 7 (11). e49553. ISSN 1932-6203 doi: 10.1371/journal.pone.0049553
• Knight, P. G., Satchell, L. and Glister, C. (2012) Intra-ovarian roles of activins and inhibins. Molecular and Cellular Endocrinology, 359 (1-2). pp. 53-65. ISSN 0303-7207 doi: 10.1016/j.mce.2011.04.024
• Holtz, W., Wang, X., El-Gayar, M. and Knight, P.G. (2012) The effect of exogenous gonadotropins on ovarian function in goats actively immunized against inhibin. Theriogenology, 77 (12). pp. 253-259. ISSN 0093-691X doi: 10.1016/j.theriogenology.2011.07.037

2011

• Glister, C., Satchell, L. and Knight, P. G. (2011) Granulosal and thecal expression of bone morphogenetic protein- and activin-binding protein mRNA transcripts during bovine follicle development and factors modulating their expression in vitro. Reproduction, 142 (4). pp. 581-591. ISSN 1741-7899 doi: 10.1530/REP-11-0150

2010

• Glister, C., Satchell, L. and Knight, P. G. (2010) Changes in expression of bone morphogenetic proteins (BMPs), their receptors and inhibin co-receptor betaglycan during bovine antral follicle development: inhibin can antagonize the suppressive effect of BMPs on thecal androgen production. Reproduction, 140 (5). pp. 699-712. ISSN 1470-1626 doi: 10.1530/REP-10-0216

2009

• Knight, P.G., Al-Musawi, S.L., Lovell, T.M. and Gladwell, R.T. (2009) Control of follicular development: intra-ovarian actions of transforming growth factor-beta (TGF-b) superfamily members. In: Hocking, P.M. (ed.) Biology of Breeding Poultry. CAB International, Wallingford, pp. 89-107. ISBN 9781845933753
• Kayani, A. R., Glister, C. and Knight, P. G. (2009) Evidence for an inhibitory role of bone morphogenetic protein(s) in the follicular-luteal transition in cattle. Reproduction, 137 (1). pp. 67-78. ISSN 1470-1626 doi: 10.1530/rep-08-0198
• Ireland, J. J., Zielak-Steciwko, A. E., Jimenez-Krassel, F., Folger, J., Bettegowda, A., Scheetz, D., Walsh, S., Mossa, F., Knight, P. G., Smith, G. W., Lonergan, P. and Evans, A. C. O. (2009) Variation in the ovarian reserve Is linked to alterations in intrafollicular estradiol production and ovarian biomarkers of follicular differentiation and oocyte quality in cattle. Biology of Reproduction, 80 (5). pp. 954-964. ISSN 0006-3363 doi: 10.1095/biolreprod.108.073791
• Wang, X. L., El-Gayar, M., Knight, P. G. and Holtz, W. (2009) The long-term effect of active immunization against inhibin in goats. Theriogenology, 71 (2). pp. 318-322. ISSN 0093-691X doi: 10.1016/j.theriogenology.2008.07.024

2008

• Veiga-Lopez, A., Ye, W., Phillips, D. J., Herkimer, C., Knight, P. G. and Padmanabhan, V. (2008) Developmental programming: Deficits in reproductive hormone dynamics and ovulatory outcomes in prenatal, testosterone-treated sheep. Biology of Reproduction, 78 (4). pp. 636-647. ISSN 0006-3363 doi: 10.1095/biolreprod.107.065904
• Butler, S. T., Pelton, S. H., Knight, P. G. and Butler, W. R. (2008) Follicle-stimulating hormone isoforms and plasma concentrations of estradiol and inhibin A in dairy cows with ovulatory and non-ovulatory follicles during the first postpartum follicle wave. Domestic Animal Endocrinology, 35 (1). pp. 112-119. ISSN 0739-7240 doi: 10.1016/j.domaniend.2008.03.002
• Ryan, K. E., Glister, C., Lonergan, P., Martin, F., Knight, P. G. and Evans, A. C. (2008) Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep. Journal of Ovarian Research, 1 (1). p. 2. ISSN 1757-2215 doi: 10.1186/1757-2215-1-2
• Padilla, G., Knight, P. G. and Holtz, W. (2008) Superovulation and embryo collection in nulliparous Boer goat does immunized against a recombinant ovine alpha-subunit inhibin. Small Ruminant Research, 74 (1-3). pp. 159-164. ISSN 0921-4488 doi: 10.1016/j.smallrumres.2007.05.006

2007

• Al-Musawi, S. L., Gladwell, R. T. and Knight, P. G. (2007) Bone morphogenetic protein-6 enhances gonadotrophin-dependent progesterone and inhibin secretion and expression of rnRNA transcripts encoding gonadotrophin receptors and inhibin/activin subunits in chicken granulosa cells. Reproduction, 134 (2). pp. 293-306. ISSN 1470-1626 doi: 10.1530/rep-07-0070
• Lovell, T. M., Al-Musawi, S. L., Gladwell, R. T. and Knight, P. G. (2007) Gonadotrophins modulate hormone secretion and steady-state mRNA levels for activin receptors (type I, IIA, IIB) and inhibin co-receptor (betaglycan) in granulosa and theca cells from chicken prehierarchical and preovulatory follicles. Reproduction, 133 (6). pp. 1159-1168. ISSN 1470-1626 doi: 10.1530/rep-06-0179

2006

• Glister, C., Groome, N. P. and Knight, P. G. (2006) Bovine follicle development is associated with divergent changes in activin-A, inhibin-A and follistatin and the relative abundance of different follistatin isoforms in follicular fluid. Journal of Endocrinology, 188 (2). pp. 215-225. ISSN 0022-0795 doi: 10.1677/joe.1.06485
• Lovell, T. M., Knight, P. G. and Gladwell, R. (2006) Differential expression of mRNAs encoding co-receptor (betaglycan) and activin type-I preovulatory and prehierarchical follicles of the putative inhibin and type-II receptors in the laying hen ovary. Journal of Endocrinology, 188 (2). pp. 241-249. ISSN 0022-0795 doi: 10.1677/joe.1.06525
• Silva, C. C., Rosa, H. J. D. and Knight, P. G. (2006) Seasonal variations in the developmental competence of bovine oocytes matured in vitro. Veterinary Record, 158 (14). pp. 473-475. ISSN 0042-4900
• Knight, P. G. and Glister, C. (2006) TGF-beta superfamily members and ovarian follicle development. Reproduction, 132 (2). pp. 191-206. ISSN 1470-1626 doi: 10.1530/rep.1.01074

2005

• Glister, C., Richards, S. L. and Knight, P. G. (2005) Bone morphogenetic proteins (BMP)-4,-6, and-7 potently suppress basal and luteinizing hormone-induced androgen production by bovine theca interna cells in primary culture: Could ovarian hyperandrogenic dysfunction be caused by a defect in thecal BMP signaling? Endocrinology, 146 (4). pp. 1883-1892. ISSN 0013-7227 doi: 10.1210/en.2004-1303
• Burns, D. S., Jimenez-Krassel, F., Ireland, J. L. H., Knight, P. G. and Ireland, J. J. (2005) Numbers of antral follicles during follicular waves in cattle: Evidence for high variation among animals, very high repeatability in individuals, and an inverse association with serum follicle-stimulating hormone concentrations. Biology of Reproduction, 73 (1). pp. 54-62. ISSN 0006-3363 doi: 10.1095/biolreprod.104.036277
• Lovell, T. M., Knight, P. G. and Gladwell, R. T. (2005) Variation in pituitary expression of mRNAs encoding the putative inhibin co-receptor (betaglycan) and type-I and type-II activin receptors during the chicken ovulatory cycle. Journal of Endocrinology, 186 (3). pp. 447-455. ISSN 0022-0795 doi: 10.1677/joe.1.06303
• Knight, P.G., Gladwell, R.T. and Lovell, T.M. (2005) The inhibin-activin system and ovarian folliculogenesis in the chicken. In: Dawson, A. and Sharp, P.J. (eds.) Functional Avian Endocrinology. Alpha Science International Ltd, New Delhi, India, pp. 323-340. ISBN 9788173195686

2004

• Bleach, E. C. L., Glencross, R. G. and Knight, P. G. (2004) Association between ovarian follicle development and pregnancy rates in dairy cows undergoing spontaneous oestrous cycles. Reproduction, 127 (5). pp. 621-629. ISSN 1470-1626 doi: 10.1530/rep.1.00190
• Glister, C., Kemp, C. F. and Knight, P. G. (2004) Bone morphogenetic protein (BMP) ligands and receptors in bovine ovarian follicle cells: actions of BMP-4,-6 and-7 on granulosa cells and differential modulation of Smad-1 phosphorylation by follistatin. Reproduction, 127 (2). pp. 239-254. ISSN 1470-1626 doi: 10.1530/rep.1.00090
• Hirst, R. C., Abel, M. H., Wilkins, V., Simpson, C., Knight, P. G., Zhang, F. P., Huhtaniemi, I., Kumar, T. R. and Charlton, H. M. (2004) Influence of mutations affecting gonadotropin production or responsiveness on expression of inhibin subunit mRNA and protein in the mouse ovary. Reproduction, 128 (1). pp. 43-52. ISSN 1470-1626 doi: 10.1530/rep.1.00176

2003

• Ryan, K. E., Glister, C., Martin, F., Knight, P. G. and Evans, A. C. O. (2003) Effect of FSH and IGF on Akt and Erk pathways, estradiol, and inhibin-A secretion, and survival/proliferation of bovine granulosa cells in vitro. Biology of Reproduction, 68. p. 423. ISSN 0006-3363
• Silva, C. C., Groome, N. P. and Knight, P. G. (2003) Immunohistochemical localization of inhibin/activin alpha, beta(A) and beta(B) subunits and follistatin in bovine oocytes during in vitro maturation and fertilization. Reproduction, 125 (1). pp. 33-42. ISSN 1470-1626 doi: 10.1530/rep.0.1250033
• Knight, P. G. and Glister, C. (2003) Local roles of TGF-beta superfamily members in the control of ovarian follicle development. Animal Reproduction Science, 78 (3-4). pp. 165-183. ISSN 0378-4320 doi: 10.1016/s0378-4320(03)00089-7
• Glister, C., Groome, N. P. and Knight, P. G. (2003) Oocyte-mediated suppression of follicle-stimulating hormone- and insulin-like growth factor-induced secretion of steroids and inhibin-related proteins by bovine granulosa cells in vitro: Possible role of transforming growth factor alpha. Biology of Reproduction, 68 (3). pp. 758-765. ISSN 0006-3363 doi: 10.1095/biolreprod.102.008698
• Lovell, T. M., Gladwell, R. T., Groome, N. P. and Knight, P. G. (2003) Ovarian follicle development in the laying hen is accompanied by divergent changes in inhibin A, inhibin B, activin A and follistatin production in granulosa and theca layers. Journal of Endocrinology, 177 (1). pp. 45-55. ISSN 0022-0795
• Anderson, G. M., Lapwood, K. R., Knight, P. G. and Parkinson, T. J. (2003) The reproductive response of rams to thyroidectomy: mediation by impaired inhibin feedback rather than a change in LH pulsatility. Reproduction, 126 (3). pp. 353-364. ISSN 1470-1626 doi: 10.1530/rep.0.1260353

This list was generated on Sat May 25 01:59:32 2013 BST.