Staff Profile:Dr Kim Watson

Name:

Dr Kim Watson

Job Title:

Academic

Responsibilities:

Areas of Interest:

Project title : Molecular recognition and transport mechanisms in biomolecular systems 

Research in my group takes a multidisciplinary approach to the study of protein structure and function, with particular focus on the use of x-ray crystallography and computational methods for structure-based and computer-aided drug discovery. The integration of advanced imaging technologies and computational science with basic molecular and cellular research offers insight into how living systems function. One of the key areas of interest in my lab is how proteins function and how that function translates to health. A detailed molecular analysis of protein-protein, and protein-ligand interactions is a crucial goal for structural biology and determination of three-dimensional protein structure is particularly important, since subtle changes in structure can affect the proper function of a protein, changing its activity, and possibly leading to its instability and ultimate destruction by the cell. Research in my group is focused on transport systems in bacteria, plants and animals; with an emphasis on metal – protein and peptide – protein interactions. Many of the protein targets of interest are potential pharmaceutical targets.

 

Research groups / Centres:

Cardiovascular biology

Publications:

Y

Jump to: 2011 | 2010 | 2008 | 2007 | 2005 | 2003

Number of items: 15.

2011

• Sarsam, S. W., Nutt, D. R., Strohfeldt, K. and Watson, K. A. (2011) Titanocene anticancer complexes and their binding mode of action to human serum albumin: a computational study. Metallomics, 3 (2). pp. 152-161. ISSN 1756-5901 doi: 10.1039/c0mt00041h

2010

• Vaiyapuri, S., Wagstaff, S. C., Watson, K. A., Harrison, R. A., Gibbins, J. M. and Hutchinson, E. G. (2010) Purification and functional characterisation of rhiminopeptidase A, a novel aminopeptidase from the venom of Bitis gabonica rhinoceros. PLoS Neglected Tropical Diseases, 4 (8). e796. ISSN 1935-2735 doi: 10.1371/journal.pntd.0000796
• Rajasekaran, M. B., Mitchell, S. A., Gibson, T. M., Hussain, R., Siligardi, G., Andrews, S. C. and Watson, K. A. (2010) Isolation and characterisation of EfeM, a periplasmic component of the putative EfeUOBM iron transporter of Pseudomonas syringae pv. syringae. Biochemical and Biophysical Research Communications, 398 (3). pp. 366-371. ISSN 0006-291X doi: 10.1016/j.bbrc.2010.06.072
• Zhou, H. G., Standfuss, J., Watson, K. A. and Krasel, C. (2010) Structure of full-length arrestin2 in a dimeric crystal form. In: 51st Annual Meeting of the Deutsche-Gesellschaft-fur-Experimentelle-und-Klinische-Pharmakologie-und-Toxikologie, 23-25 Mar 2010, Mainz, Germany.
• Rajasekaran, M. B., Nilapwar, S., Andrews, S. C. and Watson, K. A. (2010) EfeO-cupredoxins: major new members of the cupredoxin superfamily with roles in bacterial iron transport. BioMetals, 23 (1). pp. 1-17. ISSN 0966-0844 doi: 10.1007/s10534-009-9262-z

2008

• Bamford, V. A., Armour, M., Mitchell, S. A., Cartron, M., Andrews, S. C. and Watson, K. A. (2008) Preliminary X-ray diffraction analysis of YqjH from Escherichia coli: a putative cytoplasmic ferri-siderophore reductase. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 64 (9). pp. 792-796. ISSN 1744-3091 doi: 10.1107/S174430910802352X
• Pugazhendhi, D., Watson, K. A., Mills, S., Botting, N., Pope, G. S. and Darbre, P. D. (2008) Effect of sulphation on the oestrogen agonist activity of the phytoestrogens genistein and daidzein in MCF-7 human breast cancer cells. Journal of Endocrinology, 197 (3). pp. 503-515. ISSN 0022-0795 doi: 10.1677/joe-07-0384
• Baba-Dikwa, A., Thompson, D., Spencer, N. J., Andrews, S. C. and Watson, K. A. (2008) Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 64. pp. 966-969. ISSN 1744-3091 doi: 10.1107/s1744309108029515

2007

• Lamanna, C., Catalano, A., Carocci, A., Di Mola, A., Franchini, C., Tortorella, V., Vanderheyden, P. M., Sinicropi, M. S., Watson, K. A. and Sciabola, S. (2007) AT(1) receptor ligands: virtual-screening-based design with TOPP descriptors, synthesis, and biological evaluation of pyrrolidine derivatives. ChemMedChem, 2 (9). pp. 1298-310. ISSN 1860-7179 doi: 10.1002/cmdc.200700082
• Cartron, M. L., Mitchell, S., Woodhall, M. R., Andrews, S. C. and Watson, K. A. (2007) Preliminary X-ray diffraction analysis of YcdB from Escherichia coli: a novel haem-containing and Tat-secreted periplasmic protein with a potential role in iron transport. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 63. pp. 37-41. ISSN 1744-3091 doi: 10.1107/s174430910604509x
• Armour, M., Cartron, M. L., Mitchell, S. A., Bamford, V. A., Watson, K. A. and Andrews, S. C. (2007) YqjH: a novel cytopolasmic siderophore reductase found in eschericia coli. American Journal of Hematology, 82 (6). p. 582. ISSN 0361-8609

2005

• Archontis, G., Watson, K. A., Xie, Q., Andreou, G., Chrysina, E. D., Zographos, S. E., Oikonomakos, N. G. and Karplus, M. (2005) Glycogen phosphorylase inhibitors: a free energy perturbation analysis of glucopyranose spirohydantoin analogues. Proteins, 61 (4). pp. 984-98. ISSN 0887-3585
• Watson, K. A. (2005) How China and Japan are solving the protein puzzle. Global Watch, 44. pp. 4-7.
• Watson, K. A., Chrysina, E. D., Tsitsanou, K. E., Zographos, S. E., Archontis, G., Fleet, G. W. and Oikonomakos, N. G. (2005) Kinetic and crystallographic studies of glucopyranose spirohydantoin and glucopyranosylamine analogs inhibitors of glycogen phosphorylase. Proteins: Structure, Function and Bioinformatics, 61 (4). pp. 966-83. ISSN 0887-3585 doi: 10.1002/prot.20653

2003

• Browning, K., Carpenter, E., Watson, K., Coggins, J., Hawkins, A., Koch, M. and Svergun, D. (2003) Twists and turns: a tale of two shikimate-pathway enzymes. Biochemical Society Transactions, 31 (3). pp. 543-547. ISSN 1470-8752

This list was generated on Fri May 25 11:29:05 2012 BST.

 

Pre 2005

Watson KA, Johnson LNJ, Leoffen P, Gillham D, Eggleston D, Collins P, Moynihan JM, DTI government report (2004) "Structural Genomics in Japan and China" following invitation to coordinate and lead a fully funded DTI mission. The report describes the findings from a DTI funded mission to China and Japan to explore emerging HTP technologies and provide opportunities for UK collaboration/technology transfer.

Brown, K., Carpenter E., Watson, KA, Coggins, JR, Hawkins, AR, Koch, MH, Svergun, DI. (2003) "Twists and turns: a tale of two shikimate-pathway enzymes." Biochem. Soc. Trans., 31, 543-547.

Nathalie Marchand-Geneste, Kimberly A. Watson, Bjorn K. Alsberg, and Ross D. King, (2002) "New Approach to Pharmacophore Mapping and QSAR Analysis Using Inductive Logic Programming. Application to Thermolysin Inhibitors and Glycogen Phosphorylase b Inhibitors".J. Med. Chem., 45, 399-409.

Katerina Tsitsanou, Nikos G. Oikonomakos, Spiros E. Zographos, V. Skamnaki, Mary Gregoriou, Kimberly A. Watson, Louise N. Johnson, George W.J. Fleet, Protein Science, 1999, 8, 741-749. "Effect of commonly used cryoprotectants on glycogen phosphorylase activity and structure"

Kimberly A. Watson, Claire McCleverty, Silvano Geremia, Silvain Cottaz, Hughes Driguez, Louise N. Johnson, The EMBO Journal, 1999, 18, 4619. "Phosphorylase recognition and phosphorylysis of its oligosaccharide substrate: Answers to a long outstanding question"

Mark O'Reilly, Kimberly A. Watson, Louise N. Johnson, Biochemistry, 1999, 38, 5337-5345. "The crystal structure of the E.coli maltodextrin phosphorylase-acarbose complex"

Marc O'Reilly, Kimberly A. Watson, Reinhard Schinzel, Dieter Palm and Louise N. Johnson, Nature Structural Biology, 1997, 4, 405-412. "Oligosaccharide substrate binding in Escherichia coli maltodextrin phosphorylase"

Manuel Pastor, Gabriele Cruciani and Kimberly A. Watson, J. Med. Chem., 1997, 40, 4089-4102. "A Strategy for the Incorporation of Water Molecules Present in a Ligand-Binding Site into a 3D-QSAR Analysis"

Kimberly A. Watson, Reinhard Schinzel, Deiter Palm and Louise N. Johnson, The EMBO Journal, 1997, 16, 1-14. "The crystal structure of Escherichia coli maltodextrin phosphorylase provides an explanation for the activity without control in this basic archetype of a phosphorylase"