Staff Profile:Dr Ben Neuman

Name:

Dr Ben Neuman

Job Title:

Academic, AMS Building

Responsibilities:

Areas of Interest:

Research Interests:

The primary goal of my laboratory is to uncover the mechanisms by which viruses are able to co-opt host cells and organisms. This mainly involves molecular-level investigation of nulcleic acid, enzymatic and structural properties of emerging pathogens related to human diseases including SARS (coronavirus), arenavirus haemorrhagic fevers (such as Argentine and Lassa) and influenza (orthomyxovirus).

For colonization to be successful, a virus requires the ability differentiate self (viral) components from the crowded background of the cell's interior. By studying the mechanisms by which viruses are able to invade host cells and transmit genetic information, we become able to design and test specific types of antiviral intervention. We are actively collaborating with biotechnology industry firms including AVI Biopharma to develop antisense nucleic acid compounds, peptides and small molecules to combat coronavirus and arenavirus infection.

A related interest is in understanding the roles of protein and nucleic acid structure and interaction during viral assembly. In collaboration with The Scripps Research Institute, we have begun a long-term project to investigate the structure of non-helical, non-icosahedral enveloped viriuses, and structural changes that occur during entry and exit from a cell. Electron cryomicroscopy and digital image provide a unique insight into the two- and three-dimensional architecture that enables virus particles to transmit genetic information through time and space.

Research groups / Centres:

Microbiology research group

Publications:

Y

Jump to: 2013 | 2011 | 2010 | 2009 | 2008 | 2007

Number of items: 17.

2013

• Neuman, B. W., Kiss, G., Al-Mulla, H. M. N., Dokland, T., Buchmeier, M. J., Weikl, T. and Schley, D. (2013) Direct observation of membrane insertion by enveloped virus matrix proteins by phosphate displacement. PLoS ONE, 8 (2). e57916. ISSN 1932-6203

2011

• Neuman, B. W., Bederka, L. H., Stein, D. A., Ting, J. P. C., Moulton, H. M. and Buchmeier, M. J. (2011) Development of peptide-conjugated morpholino oligomers as pan-arenavirus inhibitors. Antimicrobial Agents and Chemotherapy, 55 (10). pp. 4631-4638. ISSN 1098-6596 doi: 10.1128/AAC.00650-11
• Neuman, B. W., Kiss, G., Kunding, A. H., Bhella, D., Baksh, M. F., Connelly, S., Droese, B., Klaus, J. P., Makino, S., Sawicki, S. G., Siddell, S. G., Stamou, D. G., Wilson, I. A., Kuhn, P. and Buchmeier, M. J. (2011) A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174 (1). pp. 11-22. ISSN 1047-8477 doi: 10.1016/j.jsb.2010.11.021
• Zust, R., Cervantes-Barragan, L., Habjan, M., Maier, R., Neuman, B. W., Ziebuhr, J., Szretter, K. J., Baker, S. C., Barchet, W., Diamond, M. S., Siddell, S. G., Ludewig, B. and Thiel, V. (2011) Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5. Nature Immunology, 12. pp. 137-143. ISSN 1529-2916 doi: 10.1038/ni.1979
• Stuart, A., Prescott, C., MacIntyre, S., Sethar, A., Neuman, B., McCarthy, N. D., Wimalarathna, H. and Maiden, M. C. J. (2011) The role of rodents as carriers of disease on UK farms: a preliminary investigation. In: 8th European Vertebrate Pest Management Conference, 26-30 September 2011, Berlin, Germany, pp. 198-199. (In Press) (Proceedings of the 8th European Vertebrate Pest Management Conference)

2010

• Johnson, M. A., Chatterjee, A., Neuman, B. W. and Wuthrich, K. (2010) SARS coronavirus unique domain: three-domain molecular architecture in solution and RNA binding. Journal of Molecular Biology, 400 (4). pp. 724-742. ISSN 0022-2836 doi: 10.1016/j.jmb.2010.05.027

2009

• Serrano, P., Johnson, M. A., Chatterjee, A., Neuman, B. W., Joseph, J. S., Buchmeier, M. J., Kuhn, P. and Wuthrich, K. (2009) Nuclear magnetic resonance structure of the nucleic acid-binding domain of severe acute respiratory syndrome coronavirus nonstructural protein 3. Journal of Virology, 83 (24). pp. 12998-13008. ISSN 0022-538X doi: 10.1128/jvi.01253-09
• Chatterjee, A., Johnson, M. A., Serrano, P., Pedrini, B., Joseph, J. S., Neuman, B. W., Saikatendu, K., Buchmeier, M. J., Kuhn, P. and Wuthrich, K. (2009) Nuclear magnetic resonance structure shows that the severe acute respiratory syndrome coronavirus-unique domain contains a macrodomain fold. Journal of Virology, 83 (4). pp. 1823-1836. ISSN 0022-538X doi: 10.1128/jvi.01781-08

2008

• Chatterjee, A., Johnson, M. A., Serrano, P., Pedrini, B., Joseph, J. J., Neuman, B. W., Saikatendu, K., Buchmeier, M. J., Kuhn, P. and Wuthrich, K. (2008) NMR structure shows that the SARS-unique domain contains a macrodomain fold. Journal of Virology, 83. pp. 12998-13008. ISSN 0022-538X doi: 10.1128/JVI.01781-08
• Neuman, B. W., Joseph, J. S., Saikatendu, K. S., Serrano, P., Chatterjee, A., Johnson, M. A., Liao, L., Klaus, J. P., Yates, J. R., Wuethrich, K., Stevens, R. C., Buchmeier, M. J. and Kuhn, P. (2008) Proteornics analysis unravels the functional repertoire of coronavirus nonstructural protein 3. Journal of Virology, 82 (11). pp. 5279-5294. ISSN 0022-538X doi: 10.1128/jvi.02631-07
• Neuman, B. W., Adair, B. D., Yeager, M. and Buchmeier, M. J. (2008) Purification and electron cryomicroscopy of coronavirus particles. Methods in Molecular Biology, 454. pp. 129-36. ISSN 1064-3745 doi: 10.1007/978-1-59745-181-9_12

2007

• Burrer, R., Neuman, B. W., Ting, J. P., Stein, D. A., Moulton, H. M., Iversen, P. L., Kuhn, P. and Buchmeier, M. J. (2007) Antiviral effects of antisense morpholino oligomers in murine coronavirus infection models. The Journal of Virology, 81 (11). pp. 5637-48. ISSN 0022-538X doi: 10.1128/JVI.02360-06
• Moulton, H. M., Fletcher, S., Neuman, B. W., McClorey, G., Stein, D. A., Abes, S., Wilton, S. D., Buchmeier, M. J., Lebleu, B. and Iversen, P. L. (2007) Cell-penetrating peptide-morpholino conjugates alter pre-mRNA splicing of DMD (Duchenne muscular dystrophy) and inhibit murine coronavirus replication in vivo. Biochemical Society Transactions, 35 (4). pp. 826-8. ISSN 0300-5127
• Joseph, J. S., Saikatendu, K. S., Subramanian, V., Neuman, B. W., Buchmeier, M. J., Stevens, R. C. and Kuhn, P. (2007) Crystal structure of a monomeric form of SARS-CoV endonuclease nsp15 suggests a role for hexamerization as an allosteric switch. Journal of Virology, 81 (12). pp. 6700-8. ISSN 0022-538X doi: 10.1128/JVI.02817-06
• Saunders, A. A., Ting, J. P., Meisner, J., Neuman, B. W., Perez, M., de la Torre, J. C. and Buchmeier, M. J. (2007) Mapping the landscape of the lymphocytic choriomeningitis virus stable signal peptide reveals novel functional domains. Journal of Virology, 81 (11). pp. 5649-57. ISSN 0022-538X doi: 10.1128/JVI.02759-06
• Serrano, P., Johnson, M. A., Almeida, M. S., Horst, R., Herrmann, T., Joseph, J. S., Neuman, B. W., Subramanian, V., Saikatendu, K. S., Buchmeier, M. J., Stevens, R. C., Kuhn, P. and Wuthrich, K. (2007) Nuclear magnetic resonance structure of the N-terminal domain of nonstructural protein 3 from the severe acute respiratory syndrome coronavirus. Journal of Virology, 81 (21). pp. 12049-60. ISSN 0022-538X doi: 10.1128/JVI.00969-07
• Saikatendu, K. S., Joseph, J. S., Subramanian, V., Neuman, B. W., Buchmeier, M. J., Stevens, R. C. and Kuhn, P. (2007) Ribonucleocapsid formation of severe acute respiratory syndrome coronavirus through molecular action of the N-terminal domain of N protein. Journal of Virology, 81 (8). pp. 3913-21. ISSN 0022-538X doi: 10.1128/JVI.02236-06

This list was generated on Thu May 23 01:58:47 2013 BST.

 

Pre 2007

Neuman BW, Stein DA, Kroeker AD, Moulton HM, Bestwick RK, Iversen PL,Buchmeier MJ. Inhibition and escape of SARS-CoV treated with antisense morpholino oligomers. Adv Exp Med Biol. 2006;581:567-71. Review. PubMed PMID: 17037599.

Neuman BW, Adair BD, Yoshioka C, Quispe JD, Milligan RA, Yeager M, Buchmeier MJ. Ultrastructure of SARS-CoV, FIPV, and MHV revealed by electron cryomicroscopy. Adv Exp Med Biol. 2006;581:181-5. PubMed PMID: 17037527.

Neuman BW, Adair BD, Yoshioka C, Quispe JD, Orca G, Kuhn P, Milligan RA,Yeager M, Buchmeier MJ. Supramolecular architecture of severe acute respiratorysyndrome coronavirus revealed by electron cryomicroscopy. J Virol. 2006 Aug;80(16):7918-28. PubMed PMID: 16873249; PubMed Central PMCID: PMC1563832.

Joseph JS, Saikatendu KS, Subramanian V, Neuman BW, Brooun A, Griffith M, MoyK, Yadav MK, Velasquez J, Buchmeier MJ, Stevens RC, Kuhn P. Crystal structure of nonstructural protein 10 from the severe acute respiratory syndrome coronavirus reveals a novel fold with two zinc-binding motifs. J Virol. 2006 Aug;80(16):7894-901. PubMed PMID: 16873246; PubMed Central PMCID: PMC1563791.

Saikatendu KS, Joseph JS, Subramanian V, Clayton T, Griffith M, Moy K,Velasquez J, Neuman BW, Buchmeier MJ, Stevens RC, Kuhn P. Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3. Structure. 2005 Nov;13(11):1665-75. PubMed PMID: 16271890.

Peti W, Johnson MA, Herrmann T, Neuman BW, Buchmeier MJ, Nelson M, Joseph J, Page R, Stevens RC, Kuhn P, Wüthrich K. Structural genomics of the severe acute respiratory syndrome coronavirus: nuclear magnetic resonance structure of the
protein nsP7. J Virol. 2005 Oct;79(20):12905-13. PubMed PMID: 16188992; PubMed Central PMCID: PMC1235862. 

Neuman BW, Stein DA, Kroeker AD, Churchill MJ, Kim AM, Kuhn P, Dawson P, Moulton HM, Bestwick RK, Iversen PL, Buchmeier MJ. Inhibition, escape, and attenuated growth of severe acute respiratory syndrome coronavirus treated with antisense morpholino oligomers. J Virol. 2005 Aug;79(15):9665-76. PubMed PMID:16014928; PubMed Central PMCID: PMC1181598.

Neuman BW, Adair BD, Burns JW, Milligan RA, Buchmeier MJ, Yeager M. Complementarity in the supramolecular design of arenaviruses and retroviruses revealed by electron cryomicroscopy and image analysis. J Virol. 2005 Mar;79(6):3822-30. PubMed PMID: 15731275; PubMed Central PMCID: PMC1075687.

Neuman BW, Stein DA, Kroeker AD, Paulino AD, Moulton HM, Iversen PL, Buchmeier MJ. Antisense morpholino-oligomers directed against the 5' end of the genome inhibit coronavirus proliferation and growth. J Virol. 2004 Jun;78(11):5891-9. PubMed PMID: 15140987; PubMed Central PMCID: PMC415795.