Staff Profile:Dr Andy Wetten

Name:

Dr Andy Wetten

Job Title:

Research Fellow, Harborne Building

Responsibilities:

Areas of Interest:

Plant genetic transformation:
Novel applications for Agrobacterium and biolistic-mediated gene insertion into plants are being developed and molecular and cytological analyses of gene introgression and expression are conducted in transgenic and somatic hybrid plants. These procedures are being used for the elucidation of the molecular control of flower development using the model Impatiens balsamina and the control of flower initiation and fruit production in perennial plants using Fragaria vesca and monitoring alien chromosome elimination in Solanum interspecific somatic hybrids.

Plant cryopreservation:
Both for crop germplasm collections and species of conservation interest. Protocols are under development for the cryopreservation of cocoa germplasm in somatic embryo form and I am also involved with fundamental studies concerned with understanding the basis of cryoinjury and dehydration tolerance.

Somaclonal variation:
This phenomenon can undermine the genetic fidelity of tissue culture-maintained germplasm collections and so a range of molecular tools are being employed to assess the nature and frequency of novel mutations affecting somatic embryo derived cocoa.

Research groups / Centres:

Crops research group

Publications:

Y

Jump to: 2012 | 2011 | 2010 | 2009 | 2008 | 2004 | 2003 | 1999

Number of items: 15.

2012

• Dunwell, J. and Wetten, A., eds. (2012) Transgenic plants: methods and protocols (methods in molecular biology). Methods in Molecular Biology. Humana Press, New York, pp512. ISBN 9781617795572

2011

• Vogiatzi, C., Grout, B.W.W., Wetten, A. and Toldam-Andersen, T.B. (2011) Cryopreservation of winter-dormant apple buds: 1 - variation in recovery with cultivar and winter conditions. CryoLetters, 32 (4). pp. 358-366. ISSN 0143-2044

2010

• Rodriguez-Lopez, C. M., Bravo, H. S., Wetten, A. C. and Wilkinson, M. J. (2010) Detection of somaclonal variation during cocoa somatic embryogenesis characterised using cleaved amplified polymorphic sequence and the new freeware Artbio. Molecular Breeding, 25 (3). pp. 501-516. ISSN 1380-3743 doi: 10.1007/s11032-009-9348-x
• Rodriguez-Lopez, C., Wetten, A. C. and Wilkinson, M. J. (2010) Progressive erosion of genetic and epigenetic variation in callus-derived cocoa (Theobroma cacao) plants. New Phytologist, 186 (4). pp. 856-868. ISSN 0028-646X doi: 10.1111/j.1469-8137.2010.03242.x

2009

• Adu-Gyamfi, R. and Wetten, A. (2009) Cryopreservation of cocoa (theobroma cacao L.) secondary somatic embryos by vitrification. Cryoletters, 30 (2). pp. 160-161. ISSN 0143-2044
• Fang, J. Y., Sacande, M., Pritchard, H. and Wetten, A. (2009) Influence of freezable/non-freezable water and sucrose on the viability of Theobroma cacao somatic embryos following desiccation and freezing. Plant Cell Reports, 28 (6). pp. 883-889. ISSN 0721-7714 doi: 10.1007/s00299-009-0691-5
• Fang, J. Y., Wetten, A., Adu-Gyamfi, R., Wilkinson, M. and Rodriguez-Lopez, C. (2009) Use of secondary somatic embryos promotes genetic fidelity in cryopreservation of cocoa (Theobroma cacao L.). Agricultural and Food Science, 18 (2). pp. 152-159. ISSN 1459-6067 doi: 10.2137/145960609789267579

2008

• Fang, J. Y., Wetten, A. and Johnston, J. (2008) Headspace volatile markers for sensitivity of cocoa (Theobroma cacao L.) somatic embryos to cryopreservation. Plant Cell Reports, 27 (3). pp. 453-461. ISSN 0721-7714 doi: 10.1007/s00299-007-0487-4
• Quainoo, A. K., Wetten, A. C. and Allainguillaume, J. (2008) Transmission of cocoa swollen shoot virus by seeds. Journal of Virological Methods, 150 (1-2). pp. 45-49. ISSN 0166-0934 doi: 10.1016/j.jviromet.2008.03.009
• Quainoo, A. K., Wetten, A. C. and Allainguillaume, J. (2008) The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. Journal of Virological Methods, 149 (1). pp. 91-96. ISSN 0166-0934 doi: 10.1016/j.jviromet.2008.01.007

2004

• Fang, J.-Y., Wetten, A.C. and Hadley, P. (2004) Cryopreservation of cocoa (Theobroma cacao L.) somatic embryos for long-term germplasm storage. Plant Science, 166 (3). pp. 669-675. ISSN 0168-9452 doi: 10.1016/j.plantsci.2003.11.002

2003

• Sinha, A., Wetten, A.C. and Caligari, P.D.S. (2003) Effect of biotic factors on the isolation of Lupinus albus L. protoplasts. Australian Journal of Botany, 51 (1). pp. 103-109. ISSN 0067-1924 doi: 10.1071/BT01104
• Sinha, A., Wetten, A.C. and Caligari, P.D.S. (2003) Optimization of protoplast production in white lupin. Biologia Plantarum, 47 (1). pp. 21-25. ISSN 0006-3134 doi: 10.1023/A:1027316511865
• Wilkinson, T., Wetten, A., Prychid, C. and Fay, M.F. (2003) Suitability of cryopreservation for the long-term storage of rare and endangered plant species: a case history for cosmos atrosanguineus. Annals of Botany, 91 (1). pp. 65-74. ISSN 0305-7364

1999

• de Peyer, O. S., Wetten, A. C., Dunwell, J. M. and Crabbe, M. J. C. (1999) A heterologous expression system for bovine lens transmembrane main intrinsic protein (MIP) in Nicotiana tabacum plants. Molecular vision, 5. 23. ISSN 1090-0535

This list was generated on Fri May 25 09:40:22 2012 BST.

 

Pre 2003

Rodriguez Lopez, C.M., Wetten, A.C. and Wilkinson, M.J. Detection and quantification of in vitro-culture induced chimerism using simple sequence repeat (SSR) analysis in Theobroma cacao (L.). Theor Appl Genet, 110, 2004, 157-166.

Alsheikh, M.K., Suso, H.-P., Robson, M., Battey, N.H. and Wetten, A. Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F.v. semperflorens. Plant Cell Reports, 20, 2002, 1173-1180.

Mao, A.A., Wetten, A., Fay, M.F. and Caligari, P.D.S. In vitro culture of Lilium Mackliniae Sealey, a rare endemic species. Indian J. Plant Physiol., 7, No. 3, 2002, 239-245

Richardson, K.V.A., Wetten, A.C. and Caligari, P.D.S. Cell and nuclear degradation in root meristems following exposure of potatoes (Solanum tuberosum L) to salinity, Potato Research, 44, 2002, 389-399.

Albani, M., Taylor, S., Rodriguez-Lopez, C., Cekic, C., Al-Sheikh, M., Greenland, A., Wetten, A., Wilkinson, M. and Battey, N. Fragaria vesca …One way to understand flowering in perennials. Flowering Newsletter, 31, 2001, 44-48.

de Peyer, O.S., Wetten, A.C., Dunwell, J.M. and Crabbe, M.J.C. A heterologous expression system for bovine lens transmembrane Main Intrinsic Protein (MIP) in Nicotiana tabacum plants. Molecular Vision, 5, 1999, 23-26.