Application of Technologies

Case studies:

Below we present some examples of applications to which BioCentre instruments and expertise have been successfully applied. The case studies are necessarily brief, but reflect the range of research interests of BioCentre users at UoR. Further information can be obtained by contacting the research teams or BioCentre staff involved. We will continue to add examples as pieces of work are completed.

 

Analysing the phytochemical composition of edible plants

 

Determination of 3D protein structure

 

Characterisation of plant tannins 

 

Investigation of possible toxicogenomic effects of pesticides

 

Identification of inhibitory peptides from a complex mixture

 

Quantification of DNA methylation using the Agilent 2100 BioAnalyzer  (internal)

 

Quantifying protein:protein interactions

 

Binding kinetics of an RNA binding protein

 

Detailed analysis of protein:ligand interactions

 

Posters:

Below are links to selected posters from the group of Prof Rainer Cramer which outline work performed in the BioCentre Mass Spec facility:

• Peptidomic biomarker discovery in mouse models of type 2 diabetes mellitus Poster pdf
• Serum peptide profiling using MALDI mass spectrometry Poster pdf
• Investigation of liquid MALDI and optimisation for instrument tuning and quantitative measurements Poster pdf
• Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics Poster pdf
• Equalisation of protein amounts for overcoming the high dynamic protein expression range in the analysis of plant proteomes Poster pdf
• Training the Scientists of the future (two PhD case studies) Poster pdf